CAJ | 학술논문

为对秦岭坡旱地中药材桔梗品种选育及种质资源鉴定提供依据，应用聚丙烯酰胺凝胶电泳（PAGE）技术研究了不同产地三年生桔梗不同叶位酯酶同工酶（EST）的表达差异，并通过 EST - PAGE 图谱迁移率（Rf）、联合系数（S）、酶谱相似度（T）综合分析方法，初步评价了目前在商洛坡旱地栽培的不同产地桔梗品种的亲缘关系。结果表明：6种不同产地桔梗间的亲缘关系大小顺序为：甘肃平凉＞山东淄博＞四川广元＞西安蓝田＞内蒙赤峰≈陕西商洛，内蒙赤峰与陕西商洛桔梗疑为同一品种。
위대진령파한지중약재길경품충선육급충질자원감정제공의거，응용취병희선알응효전영（PAGE）기술연구료불동산지삼년생길경불동협위지매동공매（EST）적표체차이，병통과 EST - PAGE 도보천이솔（Rf）、연합계수（S）、매보상사도（T）종합분석방법，초보평개료목전재상락파한지재배적불동산지길경품충적친연관계。결과표명：6충불동산지길경간적친연관계대소순서위：감숙평량＞산동치박＞사천엄원＞서안람전＞내몽적봉≈협서상락，내몽적봉여협서상락길경의위동일품충。
In order to provide the basis for variety breeding and germplasm identification of Chinese traditional medicine Platycodon grandiflorum in Qinling slope dryland ,platycodon esterase isozymes (EST) expression difference of different leaf position in three different regions is studied by application of polyacrylamide gel electrophoresis (PAGE) technique ,and through the EST - PAGE map mobility (Rf) ,joint coefficient (S) (T) ,enzyme spectrum similarity comprehensive analysis method ,Put forward the preliminary kinship evaluation of shangluo Platycodon grandiflorum cul-tivation varieties in the different regions of slope dryland .The results showed that the genetic relationship between differ-ent regions size order :Pingliang - Gansu > Zibo - Shandong > Guangyuan - Sichuan > Lantian - Xian > Chifeng -Neimenggou≈ Shangluo - Shaanxi ,there are no difference between Chifeng - Neimenggou and Shangluo - Shaanxi in species of the platycodonis .