CAJ | 학술논문

研究评价了茶多酚对腐败希瓦氏菌（Shewanella putrefaciens）的抑菌和杀菌效果，并初步探讨了其抑菌机理。结果表明，茶多酚对腐败希瓦氏菌的最低抑菌浓度（MIC）和最小杀菌浓度（MBC）分别为1 mg·mL-1和16 mg·mL-1，2 mg·mL-1茶多酚处理能显著抑制细菌的生长曲线，在25℃和4℃作用96 h和192 h希瓦氏菌分别下降了105 CFU·mL-1和106 CFU·mL-1，杀菌作用表现时间依赖性。茶多酚单体的杀菌能力大小分别为EGCG>ECG>EGC>EC。在茶多酚作用下，腐败希瓦氏菌胞外上清中 Na+/K+-ATP 酶和 AKP 酶活性显著上升，投射电镜（TEM）观察细菌出现了破损、变形等现象。可见，茶多酚能有效抑制腐败希瓦氏菌的生长，在低温长时间下仍表现出杀菌能力，其杀菌机制主要是引起细菌细胞壁和细胞外膜损伤，使胞内成分泄漏，导致细菌死亡。
연구평개료다다분대부패희와씨균（Shewanella putrefaciens）적억균화살균효과，병초보탐토료기억균궤리。결과표명，다다분대부패희와씨균적최저억균농도（MIC）화최소살균농도（MBC）분별위1 mg·mL-1화16 mg·mL-1，2 mg·mL-1다다분처리능현저억제세균적생장곡선，재25℃화4℃작용96 h화192 h희와씨균분별하강료105 CFU·mL-1화106 CFU·mL-1，살균작용표현시간의뢰성。다다분단체적살균능력대소분별위EGCG>ECG>EGC>EC。재다다분작용하，부패희와씨균포외상청중 Na+/K+-ATP 매화 AKP 매활성현저상승，투사전경（TEM）관찰세균출현료파손、변형등현상。가견，다다분능유효억제부패희와씨균적생장，재저온장시간하잉표현출살균능력，기살균궤제주요시인기세균세포벽화세포외막손상，사포내성분설루，도치세균사망。
Antimicrobial effect and mechanism of TP against Shewanella putrefaciens in vitro was investigated. The results showed that the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of TP against the S. putrefaciens were 1 mg·mL-1 and 16 mg·mL-1, respectively. Growth curves of S. putrefaciens were significantly inhibited in the addition of 2 mg·mL-1 TP. The population of S. putrefaciens decreased about 105 CFU·mL-1 and 106 CFU·mL-1 with the 2 mg·mL-1 TP addition after 96 h at 25℃ and 192 h at 4℃, respectively. The bactericidal activity of TP exhibited the time depending. Among the four monomer of TP, the order of bactericidal activity were EGCG>ECG>EGC>EC. The enzyme activity of Na+/K+-ATP and AKP in the culture suspension of S. putrefaciens gradually increased with the time extension in the TP treated samples. TEM images revealed that treatment of TP might lead to damage and deformation of cell. Thus, TP exhibited the good antimicrobial and bactericidal activity against S. putrefaciens by disrupting cell walls and out membrane, which cause the permeability of intracellular components and the change of cell morphology.