植物营养与肥料学报
植物營養與肥料學報
식물영양여비료학보
PLANT NUTRITION AND FERTILIZER SCIENCE
2014年
2期
280-289
,共10页
王帅%韩晓日%战秀梅%杨劲峰%王月%刘轶飞%李娜
王帥%韓曉日%戰秀梅%楊勁峰%王月%劉軼飛%李娜
왕수%한효일%전수매%양경봉%왕월%류질비%리나
玉米%氮肥%光合系统%叶绿素荧光%820 nm光吸收
玉米%氮肥%光閤繫統%葉綠素熒光%820 nm光吸收
옥미%담비%광합계통%협록소형광%820 nm광흡수
maize%nitrogen fertilization%photosynthetic system%chlorophyll fluorescence%820nm absorbance
本文在棕壤长期定位实验的基础上,测定了不同水平氮肥处理下灌浆期玉米(郑单958)穗位叶气体交换和叶绿素荧光等光合参数以及SPAD值。结果表明,施用氮肥可以引起荧光诱导曲线( OJIP曲线)显著变化,其中K、J和I点的荧光强度值明显降低;施用氮肥显著提高捕获的激子将电子传递到电子传递链中QA下游的其他电子受体的概率(Ψo)和以吸收光能为基础的性能指数(PIABS),显著降低K点的可变荧光Fk 占振幅Fo-Fj 的比例(Wk)和J点的可变荧光Fj占振幅Fo-Fp 的比例(Vj),说明PSⅡ反应中心电子传递链综合性能以及供体侧和受体侧的电子传递能力均明显提高,并且可以看出受体侧性能的改善大于供体侧;随着施氮量的增加,PSⅠ的最大氧化还原活性ΔI/Io 呈先升后降的趋势变化,说明适量施氮可以增强P700的最大氧化还原能力,并可以显著提高叶片PSⅠ与PSⅡ两个光系统之间的协调性Ф( PSⅠ/PSⅡ)。
本文在棕壤長期定位實驗的基礎上,測定瞭不同水平氮肥處理下灌漿期玉米(鄭單958)穗位葉氣體交換和葉綠素熒光等光閤參數以及SPAD值。結果錶明,施用氮肥可以引起熒光誘導麯線( OJIP麯線)顯著變化,其中K、J和I點的熒光彊度值明顯降低;施用氮肥顯著提高捕穫的激子將電子傳遞到電子傳遞鏈中QA下遊的其他電子受體的概率(Ψo)和以吸收光能為基礎的性能指數(PIABS),顯著降低K點的可變熒光Fk 佔振幅Fo-Fj 的比例(Wk)和J點的可變熒光Fj佔振幅Fo-Fp 的比例(Vj),說明PSⅡ反應中心電子傳遞鏈綜閤性能以及供體側和受體側的電子傳遞能力均明顯提高,併且可以看齣受體側性能的改善大于供體側;隨著施氮量的增加,PSⅠ的最大氧化還原活性ΔI/Io 呈先升後降的趨勢變化,說明適量施氮可以增彊P700的最大氧化還原能力,併可以顯著提高葉片PSⅠ與PSⅡ兩箇光繫統之間的協調性Ф( PSⅠ/PSⅡ)。
본문재종양장기정위실험적기출상,측정료불동수평담비처리하관장기옥미(정단958)수위협기체교환화협록소형광등광합삼수이급SPAD치。결과표명,시용담비가이인기형광유도곡선( OJIP곡선)현저변화,기중K、J화I점적형광강도치명현강저;시용담비현저제고포획적격자장전자전체도전자전체련중QA하유적기타전자수체적개솔(Ψo)화이흡수광능위기출적성능지수(PIABS),현저강저K점적가변형광Fk 점진폭Fo-Fj 적비례(Wk)화J점적가변형광Fj점진폭Fo-Fp 적비례(Vj),설명PSⅡ반응중심전자전체련종합성능이급공체측화수체측적전자전체능력균명현제고,병차가이간출수체측성능적개선대우공체측;수착시담량적증가,PSⅠ적최대양화환원활성ΔI/Io 정선승후강적추세변화,설명괄량시담가이증강P700적최대양화환원능력,병가이현저제고협편PSⅠ여PSⅡ량개광계통지간적협조성Ф( PSⅠ/PSⅡ)。
On the basis of a long-term experiment in brown soil , the photosynthetic characteristics of maize (cultivar, Zhengdan 958) ear leaves at the filling stage, SPAD value, gas exchange and chlorophyll fluorescence parameters were measured under different levels of nitrogen treatments .The results show that the OJIP Fluorescence induction curve is affected significantly by nitrogen fertilization , and the fluorescence intensity of K , J and I steps are significantly decreased .The probability that a trapped exciton moves an electron into the electron transport chain beyond QA(Ψo) and the performance index on absorption basis (PIABS) are significantly increased under the nitrogen fertilization, while the relative variable fluorescence intensity at the K-step (Wk) and the relative variable fluorescence intensity at the J-step ( Vj ) are significantly reduced .It is shown that the comprehensive performance of electron transport chain in PSⅡreaction center as well as the electron transport capacity both in the donor side and the receptor side are improved obviously .The performance of the receptor side is greater than that of the donor side.With nitrogen fertilizer levels increasing , the maximal oxidation-reduction activity of PSⅠ(ΔI/Io ) is increased first and then decreased , which indicates a moderate amount of nitrogen fertilizer could enhance the maximal oxidation-reduction ability of P 700 , and could significantly improve coordination between PS I and PSⅡoptical systems Ф( PSⅠ/PSⅡ) in maize leaves .