CAJ | 학술논문

目的探讨重组空肠弯曲菌rLTB-PEB1蛋白疫苗的免疫机制。方法经口服免疫 BALB/c 小鼠后，ELISA 法检测特异性抗体IgG 、IgA 水平；ELISPOT 法检测派伊尔结特异性抗体分泌细胞；实时荧光定量PCR 检测IFN-γ、IL-4 mRNA 表达的差异。计算疫苗保护率。结果免疫组小鼠派伊尔结特异性抗体、IFN-γ、IL-4 mRNA 水平明显增高（ P ＜0．05）。免疫组的疫苗保护率为89．3％～93．7％，可维持30周。结论重组空肠弯曲菌rLTB-PEB1蛋白疫苗口服免疫小鼠可有效预防空肠弯曲菌的感染，其免疫机制可能为诱导了TH1/TH2型免疫应答。
목적탐토중조공장만곡균rLTB-PEB1단백역묘적면역궤제。방법경구복면역 BALB/c 소서후，ELISA 법검측특이성항체IgG 、IgA 수평；ELISPOT 법검측파이이결특이성항체분비세포；실시형광정량PCR 검측IFN-γ、IL-4 mRNA 표체적차이。계산역묘보호솔。결과면역조소서파이이결특이성항체、IFN-γ、IL-4 mRNA 수평명현증고（ P ＜0．05）。면역조적역묘보호솔위89．3％～93．7％，가유지30주。결론중조공장만곡균rLTB-PEB1단백역묘구복면역소서가유효예방공장만곡균적감염，기면역궤제가능위유도료TH1/TH2형면역응답。
Objective To study the immunological response induced by Campylobacter jejuni (CJ) recom-binant protein vaccine .Methods After the oral immunization for BALB/c mice ,the levels of IgG and IgA were detected by ELISA , the specific antibody secreting cells in Payer’s patch were detected by ELISPOT , the expression levels of IFN-γ and IL-4 were detected by RT-PCR .The vaccine protection rate was calculated .Results The levels of specific antibody , IFN-γ and IL-4 were increased obviously after immuni-zation .The vaccine protection rate was 89 .3%-93 .7%( P < 0 .05) .Conclusion The oral immunization with recombinant protein vaccine may induce TH1/TH2 type immune responses and can produce protective im-munity against Campylobacter jejuni .