CAJ | 학술논문

为研究去甲斑蝥素（Norcantharidin，NCTD）对白血病细胞的DR5表达及肿瘤坏死因子相关的凋亡诱导配体（TRAIL）诱导凋亡敏感性的影响，通过流式细胞术检测NCTD和rhTRAIL作用下白血病细胞K562的凋亡、DR5表达和细胞内活性氧（reactive oxygen species，ROS）水平；通过免疫印迹技术分析激酶JNK1的表达。结果表明：NCTD以浓度依赖方式上调 DR5表达；与 rhTRAIL单独作用比较，低浓度的 NCTD（7.5μmol/L）与rhTRAIL共同处理可显著提高K562细胞凋亡百分率、ROS水平和JNK1表达。NCTD通过ROS/JNK途径上调DR5表达，增强了白血病细胞对rhTRAIL的敏感性。
위연구거갑반모소（Norcantharidin，NCTD）대백혈병세포적DR5표체급종류배사인자상관적조망유도배체（TRAIL）유도조망민감성적영향，통과류식세포술검측NCTD화rhTRAIL작용하백혈병세포K562적조망、DR5표체화세포내활성양（reactive oxygen species，ROS）수평；통과면역인적기술분석격매JNK1적표체。결과표명：NCTD이농도의뢰방식상조 DR5표체；여 rhTRAIL단독작용비교，저농도적 NCTD（7.5μmol/L）여rhTRAIL공동처리가현저제고K562세포조망백분솔、ROS수평화JNK1표체。NCTD통과ROS/JNK도경상조DR5표체，증강료백혈병세포대rhTRAIL적민감성。
To explore the effects of NCTD on DR5 expression and cell apoptosis in leukaemia cells in-duced by TRAIL,apoptosis,expression of DR5 and level of ROS of leukaemia cells K562 induced by NCTD and rhTRAIL were detected using flow cytometry,and protein kinase JNK1 was measured by west-ern blot assay .The results showed that the expression of DR5 was up-regulated by NCTD in a dose-de-pendent model .In contrast to cells induced by rhTRAIL alone,apoptosis rate,ROS and JNK1 in K562 cells treated by rhTRAIL combined with lower concentration of NCTD (7.5μmol/L)were enhanced signif-icantly.In conclusion,NCTD could sensitize leukaemia cells to rhTRAIL by up-regulation of DR5 through ROS/JNK signaling pathway .