CAJ | 학술논문

为检测细胞骨架抑制剂对狂犬病病毒（RABV）感染细胞的抑制作用，筛选了秋水仙素（Col）和细胞松弛素D（CytoD）2种骨架特异性抑制剂在人神经瘤细胞SH-SY5 Y上的适宜浓度，探讨其对RABV感染及复制的影响。结果表明：随着细胞骨架抑制剂浓度的升高，微管和微丝的破坏加剧；当Col浓度分别为0.065 mmol/L和0.130 mmol/L及CytoD浓度分别为0.25μg/mL和0.50μg/mL时，微管、微丝结构及细胞活力均与对照组无显著差异（P＞0.05），2种骨架抑制剂对RABV的感染及复制均有抑制作用。通过对RABV培养物的OD值和G基因mRNA的定量检测，发现CytoD对RABV感染的抑制作用较Col更明显，Col对RABV复制的抑制作用较CytoD更明显。
위검측세포골가억제제대광견병병독（RABV）감염세포적억제작용，사선료추수선소（Col）화세포송이소D（CytoD）2충골가특이성억제제재인신경류세포SH-SY5 Y상적괄의농도，탐토기대RABV감염급복제적영향。결과표명：수착세포골가억제제농도적승고，미관화미사적파배가극；당Col농도분별위0.065 mmol/L화0.130 mmol/L급CytoD농도분별위0.25μg/mL화0.50μg/mL시，미관、미사결구급세포활력균여대조조무현저차이（P＞0.05），2충골가억제제대RABV적감염급복제균유억제작용。통과대RABV배양물적OD치화G기인mRNA적정량검측，발현CytoD대RABV감염적억제작용교Col경명현，Col대RABV복제적억제작용교CytoD경명현。
To study the inhibitory effects of cytoskeleton inhibitors on rabies virus (RABV)infection in vitro,colchicine (Col)and cytochalasin D (CytoD)were chosen,their suitable concentration on neurob-lastoma (SH-SY5Y)was screened,and their effects on RABV earlier infection and replication were eval-uated .The results showed that aggravated damage of microtubules and actin filaments was closely associ-ated with the highly elevated concentration of inhibitors .When Col was at 0.065 mmol/L and 0.1 3 mmol/L,respectively,and CytoD at 0.25μg/mL and 0.50μg/mL,no significant structural changes of microtubules and actin were observed compared with the control (P>0.05);the two cytoskeleton in-hibitors demonstrated inhibitory ability in RABV infection and replication,RABV titer and Q-PCR of G gene mRNA confirmed that CytoD played a more important role in early RABV infection than Col,howev-er,a more significant role of the Col was proved in RABV replication than CytoD .All these results pro-vided experimental data for cytoskeleton associated RABV pathogenesis .