CAJ | 학술논문

以五年生人参根组织为试验材料，提取总RNA，反转录合成cDNA，利用RT-PCR法对人参鲨烯环氧酶（SQE）基因的cDNA进行克隆及序列分析，初步探讨人参皂苷生物合成途径中的影响因子。结果表明：获得人参SQE基因全长片段大小为1636 bp，开放阅读框长1611 bp，编码536个氨基酸残基，与其他植物核苷酸序列具有较高同源性，其中与三七、龙牙惚木、刺五加同源性分别为98％、96％、90％。
이오년생인삼근조직위시험재료，제취총RNA，반전록합성cDNA，이용RT-PCR법대인삼사희배양매（SQE）기인적cDNA진행극륭급서렬분석，초보탐토인삼조감생물합성도경중적영향인자。결과표명：획득인삼SQE기인전장편단대소위1636 bp，개방열독광장1611 bp，편마536개안기산잔기，여기타식물핵감산서렬구유교고동원성，기중여삼칠、룡아홀목、자오가동원성분별위98％、96％、90％。
Five-year ginseng root tissue was used as material .After extracting the total RNA and revers-ing transcription,Squalene Epoxidase (SQE)gene was cloned and sequenced by RT-PCR technology . Then the impact factors of ginseng saponins′biosynthetic pathway was discussed .The results showed that Panax ginseng SQE was cloned successfully,with a full length of 1 636 bp,containing a 1 61 1 bp open reading frame that encode 536 amino acids .The nucleotide showed a high homology with other plants . Furthermore,phylogenic analysis on the sequence of SQE gene with other plants showed that P .ginseng was closely related to P .Notoginseng,Alalia elata and Eleutherococcus senticosus,and the identities of nucleotide sequences reached 98%,96%and 90%,respectively .