CAJ | 학술논문

目的：对襄阳市中心医院分离1株疑似霍乱弧菌进行鉴定及药物敏感性试验，并检测其主要毒力基因。方法利用MicroScan WalkAway 40鉴定仪进行生化鉴定及药物敏感性试验，玻片凝集法确定其血清型别，应用 PCR 及测序技术分析其16SrRNA 基因；PCR 检测其6个毒力基因。结果经鉴定，该株疑似霍乱菌株为非 O1群非 O139群霍乱弧菌，经16SrRNA分析与美国国家生物技术信息中心数据库中霍乱弧菌相似性达100％。药敏试验结果显示该菌对氨苄西林、氯霉素、甲氧苄啶-磺胺甲口恶唑、四环素均敏感，毒力基因检测 rtxC 和 toxR 阳性，tcpAET、ctx A 、hlyA、tcpACL 阴性。结论该株疑似霍乱弧菌为非 O1群非 O139群霍乱弧菌，其致病与 rtxC、toxR 毒力基因有关。
목적：대양양시중심의원분리1주의사곽란호균진행감정급약물민감성시험，병검측기주요독력기인。방법이용MicroScan WalkAway 40감정의진행생화감정급약물민감성시험，파편응집법학정기혈청형별，응용 PCR 급측서기술분석기16SrRNA 기인；PCR 검측기6개독력기인。결과경감정，해주의사곽란균주위비 O1군비 O139군곽란호균，경16SrRNA분석여미국국가생물기술신식중심수거고중곽란호균상사성체100％。약민시험결과현시해균대안변서림、록매소、갑양변정-광알갑구악서、사배소균민감，독력기인검측 rtxC 화 toxR 양성，tcpAET、ctx A 、hlyA、tcpACL 음성。결론해주의사곽란호균위비 O1군비 O139군곽란호균，기치병여 rtxC、toxR 독력기인유관。
Objective To identify an suspected Vibrio cholerae isolated from Xiangyang Central Hospital and characterize the strain in terms of antibiotic resistance and relevant virulence genes.Methods Pathogen identification and susceptibility testing were completed with MicroScan WalkAway 40 Automated Microbiology System.Slide agglutination was used for serotyping. PCR and sequencing technology were employed for 16s RNA gene analysis.PCR technique was used to detect six major viru-lence genes.Results This suspectedVibrio cholerae isolate was confirmed as non-O1 and non-O139 Vibrio cholerae .Suscep-tibility testing results indicated that the strain was sensitive to ampicillin,chloramphenicol,trimethoprim-sulfamethoxazole, and tetracycline.16s RNA gene sequence analysis showed 100% homologous with the registered sequence in National Center for Biotechnology Information database.Virulence genes rtxC and toxR were identified.The other virulence genes such as tcpAET,ctxA,hlyA,and tcpACL were negative.Conclusions This suspected Vibrio cholerae isolate is confirmed as non-O1 and non-O139 Vibrio cholerae .The pathogenic factors may be related to the virulence genes rtxC and toxR.