CAJ | 학술논문

目的：研究小鼠单侧耳蜗损毁后不同时间点耳蜗核（cochlear nucleus ，CN ）和下丘（inferior collicu-lus ，IC）生长相关蛋白-43（growth associated protein -43，GAP-43）的表达变化，探讨小鼠脑干听觉中枢的可塑性。方法将成年健康小鼠30只随机分为耳蜗损毁术后3、7、15、30、60天组及对照组，每组5只。前5组制成单侧耳蜗损伤模型，对照组仅行耳后切口、缝合，不损毁耳蜗。采用免疫组化SP法检测各组耳蜗核和下丘GAP -43蛋白的表达变化。结果①小鼠单侧耳蜗损毁后3、7、15天，术侧耳蜗核GAP-43较对照组表达升高（P＜0．05）；术后30天，术侧GAP-43表达水平下降，但仍高于对照组及对侧（均 P＜0．05）；术后60天，术侧GAP-43表达水平与对照组差异无统计学意义（P＞0．05）；对侧耳耳蜗核GAP -43含量水平与对照组差异均无统计学意义（P＞0．05）。②小鼠单侧耳蜗损毁后3、7、15天，术耳同侧和对侧下丘G A P-43表达水平较对照组升高，且表达均持续上升，差异有统计学意义（P＜0．05），术耳同侧表达水平低于对侧（P＜0．05）；术后30天表达逐渐下降，但术耳同侧和对侧仍高于正常对照组（P＜0．05），且术侧低于对侧（P＜0．05）；术后60天其表达继续下降，术耳同侧及对侧与对照组、术耳同侧与对侧表达差异均无统计学意义（均 P＞0．05）。结论单侧耳蜗损毁后，小鼠耳蜗核和下丘核GAP-43的表达先升高后下降，可能反映了脑干听觉中枢神经元在听力损失后轴突的再生及突触重塑。
목적：연구소서단측이와손훼후불동시간점이와핵（cochlear nucleus ，CN ）화하구（inferior collicu-lus ，IC）생장상관단백-43（growth associated protein -43，GAP-43）적표체변화，탐토소서뇌간은각중추적가소성。방법장성년건강소서30지수궤분위이와손훼술후3、7、15、30、60천조급대조조，매조5지。전5조제성단측이와손상모형，대조조부행이후절구、봉합，불손훼이와。채용면역조화SP법검측각조이와핵화하구GAP -43단백적표체변화。결과①소서단측이와손훼후3、7、15천，술측이와핵GAP-43교대조조표체승고（P＜0．05）；술후30천，술측GAP-43표체수평하강，단잉고우대조조급대측（균 P＜0．05）；술후60천，술측GAP-43표체수평여대조조차이무통계학의의（P＞0．05）；대측이이와핵GAP -43함량수평여대조조차이균무통계학의의（P＞0．05）。②소서단측이와손훼후3、7、15천，술이동측화대측하구G A P-43표체수평교대조조승고，차표체균지속상승，차이유통계학의의（P＜0．05），술이동측표체수평저우대측（P＜0．05）；술후30천표체축점하강，단술이동측화대측잉고우정상대조조（P＜0．05），차술측저우대측（P＜0．05）；술후60천기표체계속하강，술이동측급대측여대조조、술이동측여대측표체차이균무통계학의의（균 P＞0．05）。결론단측이와손훼후，소서이와핵화하구핵GAP-43적표체선승고후하강，가능반영료뇌간은각중추신경원재은력손실후축돌적재생급돌촉중소。
Objective To study the changes of the expression of growth associated protein -43(GAP 43) in cochlear nucleus and the inferior colliculus (IC) in rats after unilateral cochlear damage at different time points after surgery ,and explore the remodeling and repair process of synapse in brainstem auditory center of rats after auditory deprivation .Methods Thirty rats were randomly divided into six groups :the 3 ,7 ,15 ,30 ,60 days group (with u-nilateral cochlea damage) and the control group (without cochlea damage) with 5 rats in each group .The immuno-histochemical SP method was used to detect the expression of GAP -43 in cochlear nucleus and inferior colliculus at different time points after cochlea damaged in the control group .Results After the unilateral cochlear ear was dam-aged at 3 ,7 ,15 days ,GAP -43 in operation side cochlear nucleus were expressed and the expression level signifi-cantly increased compared with normal control group .After 30 days postoperatively ,GAP - 43 expression level decreased ,but it was still higher than normal control group .After 60 days postoperatively ,GAP - 43 expression level was slightly higher than that of normal control group .After 3 days postoperatively ,GAP - 43 expression level was higher than normal control group .After 7 ,15 days postoperatively ,expression levels were increased .After 30 , 60 days postoperatively ,the expressions gradually decreased ,but still higher than the normal control group .Conclusion After unilateral cochlea damaged ,GAP - 43 was expressed an obvious dynamic change process in cochlear nucleus and infe-rior colliculus .It may reflect the axonal regeneration and synaptic plasticity after hearing loss in brainstem auditory neurons .