听力学及言语疾病杂志
聽力學及言語疾病雜誌
은역학급언어질병잡지
JOURNAL OF AUDIOLOGY AND SPEECH PATHOLOGY
2014年
3期
286-289
,共4页
水通道蛋白2%醋酸去氨加压素%内淋巴积水%耳蜗
水通道蛋白2%醋痠去氨加壓素%內淋巴積水%耳蝸
수통도단백2%작산거안가압소%내림파적수%이와
Aquaporin 2%Deamino arginine vasopressin%Endolymphatic hydrops%Cochlea
目的：观察醋酸去氨加压素（deamino arginine vasopressin ，DDAVP）对内淋巴积水模型豚鼠耳蜗水通道蛋白2（aquapo rins 2，A Q P2）表达的调节，探讨梅尼埃病的发病机制。方法40只成年豚鼠随机分为实验组和对照组，每组各20只。实验组豚鼠腹腔注射DDAVP 4μg · kg -1· d-1，共7天，构建内淋巴积水模型，对照组豚鼠腹腔注射等量生理盐水。停药7天后分别取两组各5只豚鼠耳蜗切片行 HE染色，计算中阶与中阶加前庭阶横截面积比值平均值（R值）评估内淋巴积水程度，同时，2组各15只豚鼠分别采用免疫组化法和蛋白印迹法检测耳蜗A Q P2蛋白的表达。结果实验组和对照组的R值分别为0．42±0．04和0．31±0．03，实验组豚鼠内淋巴积水程度重于对照组（P＜0．05）；实验组和对照组豚鼠耳蜗AQP2均表达于血管纹、螺旋神经节细胞，其中，实验组耳蜗A Q P2蛋白半定量分析（0．9954±0．0498）高于对照组（0．7664±0．1452），差异有统计学意义（ P＜0．05）。结论DDAVP上调豚鼠耳蜗中与水转运密切相关的AQP2的基因表达，参与内淋巴积水形成。
目的：觀察醋痠去氨加壓素（deamino arginine vasopressin ，DDAVP）對內淋巴積水模型豚鼠耳蝸水通道蛋白2（aquapo rins 2，A Q P2）錶達的調節，探討梅尼埃病的髮病機製。方法40隻成年豚鼠隨機分為實驗組和對照組，每組各20隻。實驗組豚鼠腹腔註射DDAVP 4μg · kg -1· d-1，共7天，構建內淋巴積水模型，對照組豚鼠腹腔註射等量生理鹽水。停藥7天後分彆取兩組各5隻豚鼠耳蝸切片行 HE染色，計算中階與中階加前庭階橫截麵積比值平均值（R值）評估內淋巴積水程度，同時，2組各15隻豚鼠分彆採用免疫組化法和蛋白印跡法檢測耳蝸A Q P2蛋白的錶達。結果實驗組和對照組的R值分彆為0．42±0．04和0．31±0．03，實驗組豚鼠內淋巴積水程度重于對照組（P＜0．05）；實驗組和對照組豚鼠耳蝸AQP2均錶達于血管紋、螺鏇神經節細胞，其中，實驗組耳蝸A Q P2蛋白半定量分析（0．9954±0．0498）高于對照組（0．7664±0．1452），差異有統計學意義（ P＜0．05）。結論DDAVP上調豚鼠耳蝸中與水轉運密切相關的AQP2的基因錶達，參與內淋巴積水形成。
목적：관찰작산거안가압소（deamino arginine vasopressin ，DDAVP）대내림파적수모형돈서이와수통도단백2（aquapo rins 2，A Q P2）표체적조절，탐토매니애병적발병궤제。방법40지성년돈서수궤분위실험조화대조조，매조각20지。실험조돈서복강주사DDAVP 4μg · kg -1· d-1，공7천，구건내림파적수모형，대조조돈서복강주사등량생리염수。정약7천후분별취량조각5지돈서이와절편행 HE염색，계산중계여중계가전정계횡절면적비치평균치（R치）평고내림파적수정도，동시，2조각15지돈서분별채용면역조화법화단백인적법검측이와A Q P2단백적표체。결과실험조화대조조적R치분별위0．42±0．04화0．31±0．03，실험조돈서내림파적수정도중우대조조（P＜0．05）；실험조화대조조돈서이와AQP2균표체우혈관문、라선신경절세포，기중，실험조이와A Q P2단백반정량분석（0．9954±0．0498）고우대조조（0．7664±0．1452），차이유통계학의의（ P＜0．05）。결론DDAVP상조돈서이와중여수전운밀절상관적AQP2적기인표체，삼여내림파적수형성。
Objective To investigate the expression of aquaporin 2 (AQP2) in an endolymphatic hydrops ani-mal model .Methods Forty adult guinea pigs were randomly divided into experiment group and control group (20 animals in each group ) . Guinea pigs in the experiment group were treated with deamino arginine vasopressin (DDAVP) intraperitoneally at the dosage of 4 μg · kg -1 · d-1 for 7 days ,while those in the control group were treated with physiological saline .The endolymphatic hydrops and expression of AQP2 in cochleas of all animals were evaluated by Hematoxylin and Eosin stain ,immunohistochemistry and Western blot .Results The endolymphatic hydrops in the experiment group were more severe than that in the control group(P<0 .05) .Expression of AQP2 were found in the stria vascularis and spiral ganglions in both groups with similar patterns .Both immunohistochem-istry and Western blot showed that the expressions of AQP2 in the experiment group were significantly stronger(P<0 .05 ) .Conclusion DDAVP can up-regulate the expression of AQP2 in the cochleae of guinea pigs ,thus may be closely related to the pathogenesis of endolymphatic hydrops .
