CAJ | 학술논문

采用常规生物急性毒性试验法，探讨了敌敌畏对克氏原螯虾（Procambarus clarkii）成虾的急性毒性，并进一步研究了不同质量浓度（0、0．385、0．496、0．638 mg/L）敌敌畏胁迫条件下，对克氏原螯虾（Procambarus clarkii）肝胰腺T-SOD、CAT、ACP、AKP及肌肉Na＋K＋-ATPase、TChE 活性的影响．试验结果表明，正常溶氧条件下，敌敌畏对克氏原螯虾成虾的24、48、72和96 h LC50分别为1．79、1．44、1．25和0．98 mg/L，安全质量浓度（SC）为0．28 mg/L；在敌敌畏胁迫下，4 d后受试克氏原螯虾肝胰腺T-SOD和CAT都明显的被抑制下降；随着敌敌畏浓度的升高，肝胰腺组织ACP活性升高，而AKP活性表现为先抑制后诱导，肌肉TCHE和Na＋K＋-ATPase活性均受抑制．
채용상규생물급성독성시험법，탐토료활활외대극씨원오하（Procambarus clarkii）성하적급성독성，병진일보연구료불동질량농도（0、0．385、0．496、0．638 mg/L）활활외협박조건하，대극씨원오하（Procambarus clarkii）간이선T-SOD、CAT、ACP、AKP급기육Na＋K＋-ATPase、TChE 활성적영향．시험결과표명，정상용양조건하，활활외대극씨원오하성하적24、48、72화96 h LC50분별위1．79、1．44、1．25화0．98 mg/L，안전질량농도（SC）위0．28 mg/L；재활활외협박하，4 d후수시극씨원오하간이선T-SOD화CAT도명현적피억제하강；수착활활외농도적승고，간이선조직ACP활성승고，이AKP활성표현위선억제후유도，기육TCHE화Na＋K＋-ATPase활성균수억제．
Acute Toxicity of dichlorovos to Procambarus clarkiia dult was studied using the method of usual biological acute toxic test.The crayfish was exposed in 0,0.385,0.496 and 0.638 mg/L of dichlorovos solution after 4 days,respectively.Four nonspecific immune factors (T-SOD、CAT、ACP and AKP)in hepatopancreas and two (Na+K+ -ATPases and TChE ) in muscle were detected.The results indicated that the half lethal concentrations of 24,48,72,96 h of dichlorovos on Procambarus clarkiia adult were respectively 1 .79,1 .44,1 .25 and 0.98 mg/L,and the safe concentrations of nitrite nitrogen was 0.28 mg/L at normal oxygen level.The activity of T-SOD and CAT in hepatopancreas under the stress of dichlorovos nitrogen were significantly lower(P$0.05) compared with control group,and the effects of dichlorovos on Na+K+-ATPases and TChE in muscle had the same varying trend as T-SOD and CAT in hepatopancreas.The levels of ACP activities in hepatopancreas were induced significantly after 4 days with the concentration increasing,but AKP activities first declined,then increased.