CAJ | 학술논문

目的：研究苍附导痰丸加减对多囊卵巢综合征（ PCOS）大鼠卵巢形态及性激素的影响，探讨苍附导痰丸加减治疗PCOS的机制。方法：雌性大鼠随机分成5组，每组大鼠10只。模型组：采用丙酸睾酮＋绒毛膜促性腺激素（ HCG）建立PCOS大鼠病理模型；中药高剂量组：造模结束后苍附导痰丸加减24 g/kg灌胃；中药低剂量组：造模结束后苍附导痰丸加减6 g/kg灌胃；克罗米芬组：造模结束后，第1～14天灌胃给予克罗米芬悬液；空白对照组：未造膜大鼠颈背部皮下注射等量的生理盐水及继予灌胃等量生理盐水。14天后光镜观察卵巢大体形态变化，卵泡发育情况，放射免疫法测定血清卵泡刺激素（FSH）、雄激素（T）、雌二醇（E2）、促黄体生成素（LH）水平。结果：中药组用药后大鼠体质量及卵巢重量减轻，以高剂量组明显；中药高剂量组能显著降低大鼠血清中T、LH水平，增加E2、FSH水平（ P＜0.05）。结论：苍附导痰丸加减对丙酸睾酮＋HCG建立的PCOS大鼠有一定的治疗作用，可改善卵巢功能及性激素水平异常。
목적：연구창부도담환가감대다낭란소종합정（ PCOS）대서란소형태급성격소적영향，탐토창부도담환가감치료PCOS적궤제。방법：자성대서수궤분성5조，매조대서10지。모형조：채용병산고동＋융모막촉성선격소（ HCG）건립PCOS대서병리모형；중약고제량조：조모결속후창부도담환가감24 g/kg관위；중약저제량조：조모결속후창부도담환가감6 g/kg관위；극라미분조：조모결속후，제1～14천관위급여극라미분현액；공백대조조：미조막대서경배부피하주사등량적생리염수급계여관위등량생리염수。14천후광경관찰란소대체형태변화，란포발육정황，방사면역법측정혈청란포자격소（FSH）、웅격소（T）、자이순（E2）、촉황체생성소（LH）수평。결과：중약조용약후대서체질량급란소중량감경，이고제량조명현；중약고제량조능현저강저대서혈청중T、LH수평，증가E2、FSH수평（ P＜0.05）。결론：창부도담환가감대병산고동＋HCG건립적PCOS대서유일정적치료작용，가개선란소공능급성격소수평이상。
Objective:To investigate the effect of traditional Chinese medicine modified TCM -CFDTW on ovary tissues pathology and improving sex hormone in rat models of PCOS .Methods:Rat models of PCOS were es-tablished by the combined administration of testosterone propionate with human chorionic gonadotropin .After the confirmation of successful establishment of PCOS ,clomiphene and two different doses TCM -CFDTW de-coction 24g/kg,6g/kg were taken respectively by intragastric administration for 14 days.Following the collec-tion of various samples ,the rats’ weight and the ovarian weight were dertermined , general morphology of rat o-vary and development of follicles were observed under light microscopy .Blood levels of androgen , estradiol , luteinizing hormone ( LH ) , follicle stimulating hormone ( FSH ) were determined by radioimmunoassay .Re-sults:TCM-CFDTW treatment with higher dosage significantly decreased the rats ’ weight and ovarian weight , and circulating levels of androgen , LH.In addition, higher dosage of TCM -CFDTW increased blood levels of estradiol,FSH.It also inceased the follicular wall pellet cellular layer and yellow body quantity .However, TCM-CFDTW treatment at lower dosage did not exhibit a significant efficacy .Conclusion:Modified TCM-CFDTW shows significantly therapeutic efficacy on PCOS in rat models .This effect of TCM-CFDTW mainly depends on improving PCO situation and improving sex hormone .