辽宁医学院学报
遼寧醫學院學報
료녕의학원학보
JOURNAL OF LIAONING MEDICAL UNIVERSITY
2014年
3期
1-3,7
,共4页
NSCLC%HPV%Rb蛋白%Mcl-1蛋白
NSCLC%HPV%Rb蛋白%Mcl-1蛋白
NSCLC%HPV%Rb단백%Mcl-1단백
NSCLC%HPV%Rb protein%Mcl-1 protein
目的探讨人乳头瘤病毒( Human papillomavirus, HPV)感染, Rb蛋白和Mcl-1蛋白表达的变化同非小细胞肺癌(Non-small-cell lung cancer, NSCLC)的相关性。方法分别应用PCR、免疫组化方法(IHC)检测NSCLC组(79例)和肺良性病变组(11例)中HPV DNA表达和Rb蛋白、 Mcl-1蛋白的表达。结果在NSCLC组织中, HPV DNA的PCR检测阳性检出率为46.8%,肺良性病变组阳性检出率为9.1%( P<0.05), HPV阳性表达与组织学类型、分化程度及吸烟相关。 NSCLC中HPV阳性组Rb蛋白表达阳性率(37.8%)显著低于阴性组(61.9%)(P<0.05)。 NSCLC中HPV阳性组和阴性组Mcl-1蛋白表达阳性率分别为59.5%和35.7%(P<0.05)。肺良性病变组中Rb蛋白表达阳性率为72.7%而Mcl-1蛋白未表达。结论 NSCLC的发生发展可能与HPV感染后通过抑制Rb蛋白通路同时促进Mcl-1蛋白通路表达有关。
目的探討人乳頭瘤病毒( Human papillomavirus, HPV)感染, Rb蛋白和Mcl-1蛋白錶達的變化同非小細胞肺癌(Non-small-cell lung cancer, NSCLC)的相關性。方法分彆應用PCR、免疫組化方法(IHC)檢測NSCLC組(79例)和肺良性病變組(11例)中HPV DNA錶達和Rb蛋白、 Mcl-1蛋白的錶達。結果在NSCLC組織中, HPV DNA的PCR檢測暘性檢齣率為46.8%,肺良性病變組暘性檢齣率為9.1%( P<0.05), HPV暘性錶達與組織學類型、分化程度及吸煙相關。 NSCLC中HPV暘性組Rb蛋白錶達暘性率(37.8%)顯著低于陰性組(61.9%)(P<0.05)。 NSCLC中HPV暘性組和陰性組Mcl-1蛋白錶達暘性率分彆為59.5%和35.7%(P<0.05)。肺良性病變組中Rb蛋白錶達暘性率為72.7%而Mcl-1蛋白未錶達。結論 NSCLC的髮生髮展可能與HPV感染後通過抑製Rb蛋白通路同時促進Mcl-1蛋白通路錶達有關。
목적탐토인유두류병독( Human papillomavirus, HPV)감염, Rb단백화Mcl-1단백표체적변화동비소세포폐암(Non-small-cell lung cancer, NSCLC)적상관성。방법분별응용PCR、면역조화방법(IHC)검측NSCLC조(79례)화폐량성병변조(11례)중HPV DNA표체화Rb단백、 Mcl-1단백적표체。결과재NSCLC조직중, HPV DNA적PCR검측양성검출솔위46.8%,폐량성병변조양성검출솔위9.1%( P<0.05), HPV양성표체여조직학류형、분화정도급흡연상관。 NSCLC중HPV양성조Rb단백표체양성솔(37.8%)현저저우음성조(61.9%)(P<0.05)。 NSCLC중HPV양성조화음성조Mcl-1단백표체양성솔분별위59.5%화35.7%(P<0.05)。폐량성병변조중Rb단백표체양성솔위72.7%이Mcl-1단백미표체。결론 NSCLC적발생발전가능여HPV감염후통과억제Rb단백통로동시촉진Mcl-1단백통로표체유관。
Objective To study the relationship between HPV ( Human papillomavirus, HPV) infection, the expression of Rb&Mcl-1 protein and the non-small cell lung cancer ( Non-small-cell Lung Cancer, NSCLC) . Methods PCR and Immunohistochemis-try (IHC) were used to detect HPV DNA expression and protein expression of Rb and Mcl-1 in NSCLC group (79 cases) and benign lung lesion group (11 cases). Results The positive rate of HPV in NSCLC group is 46.8% , and the positive rate of HPV in lung benign lesion group is 9.1% (P<0.05). HPV positive expression is related to histological type, degree of differentiation and smoking. Rb protein positive expression in HPV-positive group ( 37.8%) is significantly lower than the negative group ( 61.9%) in NSCLC ( P<0.05 ) . Mcl-1 protein positive expression in HPV-positive group ( 59.5%) is significantly lower than the negative group (35.7%) in NSCLC (P<0.05). In benign lesion group, the rate of Rb protein expression is 72.7%, but Mcl-1 protein is not ex-pressed. Conclusion Development of NSCLC is related to HPV infection by inhibiting the Rb protein pathway and promoting Mcl-1 protein pathway.
