CAJ | 학술논문

目的探讨miR-34a在人鼻咽癌CNE2细胞中的生物学功能。方法采用MTT、Transwell侵袭实验、细胞划痕实验分别检测过表达miR-34a对人鼻咽癌CNE2细胞的生长与侵袭作用。结果 MTT检测发现,过表达miR-34a可抑制人鼻咽癌CNE2细胞的生长增殖；细胞划痕和Transwell侵袭实验显示,在鼻咽癌CNE2细胞中过表达miR-34a 48 h后划痕愈合率为40.3%依9.0%,与对照组(76.3%依6.8%)比较,能明显减缓CNE2细胞划痕的愈合,转染miR-34a mimics 48 h后穿过基底膜的细胞数为71依10,与对照组(147依5)比较,能明显减缓CNE2细胞侵袭能力(P<0.01)。结论 miR-34a可抑制鼻咽癌CNE2细胞生长与侵袭。
목적탐토miR-34a재인비인암CNE2세포중적생물학공능。방법채용MTT、Transwell침습실험、세포화흔실험분별검측과표체miR-34a대인비인암CNE2세포적생장여침습작용。결과 MTT검측발현,과표체miR-34a가억제인비인암CNE2세포적생장증식；세포화흔화Transwell침습실험현시,재비인암CNE2세포중과표체miR-34a 48 h후화흔유합솔위40.3%의9.0%,여대조조(76.3%의6.8%)비교,능명현감완CNE2세포화흔적유합,전염miR-34a mimics 48 h후천과기저막적세포수위71의10,여대조조(147의5)비교,능명현감완CNE2세포침습능력(P<0.01)。결론 miR-34a가억제비인암CNE2세포생장여침습。
Objective To analyse the mechanism of the role of miR-34a in the nasopharyngeal carcinoma cells.Methods Cell growth was evaluated by MTT. Cell invasion potential was evaluated by scratch test and transwell assay.Results The MTT assay demonstrated that after overexpressing miR-34a in nasopharyngeal carcinoma cells,cellular viabil-ity was significantly reduced (P<0. 05). The Transwell invasion chamber assay illustrated that after increasing the expres-sion of miR-34a,the number of cells passing through the Transwell chamber was significantly reduced (P<0. 01). Con-clusions miR-34a can inhibit nasopharyngeal carcinoma cell proliferation,invasion and induced apoptosis.