CAJ | 학술논문

目的：研究淫羊藿对早期兔膝骨性关节炎模型软骨的影响．方法：将18只新西兰大白兔随机取6只为正常组，12只制作兔膝骨性关节炎模型；2周后待兔膝骨性关节炎模型建立，均行MRI检查，再将12只造模兔随机分为模型组6只、药物组6只．第15日起每天开始予药物组淫羊藿按质量分数为1 g/kg灌胃，模型组及正常组则给予等量蒸馏水，所有实验兔灌胃4周后，再行MRI检查，测量信噪比（SNR）、T1ρ值，取胫骨平台用实时PCR测定软骨中MMP-3mRNA表达水平，股骨远端行HE和番红-固绿染色．结果：造模2周后，造模组SNR值低于正常组（P＜0.05），T1ρ值高于正常组（P＜0.01）；经治疗后，药物组SNR值高于模型组（P＜0.01），T1ρ值低于模型组（P＜0.05）．模型组较正常组MMP-3mRNA表达水平增加（P＜0.05），关节软骨组织结构破坏严重，软骨细胞外基质中蛋白多糖减少；药物组较模型组MMP-3mRNA表达水平下降（P＜0.05），软骨结构破坏减轻，蛋白多糖丢失明显减少．结论：淫羊藿可减缓膝骨性关节炎的软骨退变，可能与减少蛋白多糖的丢失、下调软骨中MMP-3的表达有关．
목적：연구음양곽대조기토슬골성관절염모형연골적영향．방법：장18지신서란대백토수궤취6지위정상조，12지제작토슬골성관절염모형；2주후대토슬골성관절염모형건립，균행MRI검사，재장12지조모토수궤분위모형조6지、약물조6지．제15일기매천개시여약물조음양곽안질량분수위1 g/kg관위，모형조급정상조칙급여등량증류수，소유실험토관위4주후，재행MRI검사，측량신조비（SNR）、T1ρ치，취경골평태용실시PCR측정연골중MMP-3mRNA표체수평，고골원단행HE화번홍-고록염색．결과：조모2주후，조모조SNR치저우정상조（P＜0.05），T1ρ치고우정상조（P＜0.01）；경치료후，약물조SNR치고우모형조（P＜0.01），T1ρ치저우모형조（P＜0.05）．모형조교정상조MMP-3mRNA표체수평증가（P＜0.05），관절연골조직결구파배엄중，연골세포외기질중단백다당감소；약물조교모형조MMP-3mRNA표체수평하강（P＜0.05），연골결구파배감경，단백다당주실명현감소．결론：음양곽가감완슬골성관절염적연골퇴변，가능여감소단백다당적주실、하조연골중MMP-3적표체유관．
Aim:To investigate the effect of epimedium in cartilage of osteoarthritic knee of rabbits in the early stage.Methods:Six rabbits were selected randomly from 1 8 rabbits as normal group,and the rest 1 2 rabbits as control group were made into knee osteoarthritis models.After 2 weeks,all the rabbits were examined by MRI.The 1 2 rabbits with knee osteoarthritis were then randomly divided into 2 groups:model group and epimedium group.The rabbits in epimedium group were received a daily administration of epimedium at a dose of 1 g·kg-1 ·d -1 of body weight from the 1 5 th day.The other rabbits were re-ceived distilled water of the same volume.All the rabbits were examined by MRI and measured the values of signal to noise ratio (SNR)and T1ρagain after 4 weeks treatment.Then all the rabbits were sacri-ficed.Real time PCR was used to detect the expression level of MMP-3 mRNA in cartilage with the tibia platform of right knee.The samples of right femora were stained by HE and safranin-fast green.Results:After two weeks,the SNR value in control group was lower than that in normal group(P<0.05 ).The T1ρvalue in control group was higher than that in normal group (P <0.01 ).Compared with model group,the SNR value was increased(P<0.01 ),the T1ρvalue was decreased (P<0.05)in epimedium group,after 4 weeks treatment.Compared with normal group,the expression level of MMP-3mRNA was increased in model group (P<0.05 )relatively.At the same time,the structure of cartilage was de-stroyed and proteoglycan in cartilage extracellular matrix was decreased in model group.The expression level of MMP-3 mRNA in epimedium group was decreased more significantly compared with that in model group(P<0.05 ).Compared with model group,the proteoglycan was less decreased,and the degree of pathological changes of cartilage showed the lighter destruction in epimedium group.Conclusion:Epime-dium can slow down the degeneration of cartilage of knee osteoarthritis.It might be through preventing loss of proteoglycan in cartilage extracellular matrix,inducing the expression of MMP-3 in cartilage.