CAJ | 학술논문

以鸢尾属植物黄菖蒲（Iris pseudacorus L．）为材料，研究不同比例Pb尾矿渗出液作为栽培基质对黄菖蒲幼苗的生长与生理指标的影响．结果表明，随着Pb尾矿渗出液体积分数不断升高，黄菖蒲幼苗各项生长指标都有一定程度的下降，在Pb尾矿渗出液体积分数为75％时，地上和地下部的干质量与对照相比差异显著（P＜0.05）．在Pb尾矿渗出液体积分数为100％处理条件下，叶绿素a、b和类胡萝卜素质量分数较对照相比分别下降了42％、46％和52％．所有处理黄菖蒲地下及地上部相对电导率均高于对照．而地上和地下部的超氧化物歧化酶（SOD）活性都随Pb尾矿渗出液体积分数的升高而增加；在100％Pb尾矿渗出液胁迫下，地上和地下部过氧化物酶（POD）活性上升最显著（P＜0.05），分别是对照的1.2倍和1.5倍．地上及地下部脯氨酸（Pro）质量分数最高值都出现在Pb液体积分数为100％处理中，均与对照差异显著（P＜0.05）．黄菖蒲地上和地下部分的抗坏血酸（AsA）质量分数随Pb液体积分数的提高呈下降的趋势．试验结果表明，黄菖蒲在Pb尾矿渗出液胁迫下有一定的生理抗性，黄菖蒲能够用于Pb尾矿渗出液及其污染环境的修复．
이연미속식물황창포（Iris pseudacorus L．）위재료，연구불동비례Pb미광삼출액작위재배기질대황창포유묘적생장여생리지표적영향．결과표명，수착Pb미광삼출액체적분수불단승고，황창포유묘각항생장지표도유일정정도적하강，재Pb미광삼출액체적분수위75％시，지상화지하부적간질량여대조상비차이현저（P＜0.05）．재Pb미광삼출액체적분수위100％처리조건하，협록소a、b화류호라복소질량분수교대조상비분별하강료42％、46％화52％．소유처리황창포지하급지상부상대전도솔균고우대조．이지상화지하부적초양화물기화매（SOD）활성도수Pb미광삼출액체적분수적승고이증가；재100％Pb미광삼출액협박하，지상화지하부과양화물매（POD）활성상승최현저（P＜0.05），분별시대조적1.2배화1.5배．지상급지하부포안산（Pro）질량분수최고치도출현재Pb액체적분수위100％처리중，균여대조차이현저（P＜0.05）．황창포지상화지하부분적항배혈산（AsA）질량분수수Pb액체적분수적제고정하강적추세．시험결과표명，황창포재Pb미광삼출액협박하유일정적생리항성，황창포능구용우Pb미광삼출액급기오염배경적수복．
TheseedlinggrowthandphysiologicalresponseofIrispseudacorusL.werestudiedunder the stress of Pb tailings leaching liquid (PbL)by pot culture.The results showed that the growth indexes of I.pseudacorus decreased gradually as the level of Pb tailing leaching liquid increased and aboveground and underground dry weight under 75% Pb tailing leaching liquid concentration was significantly lower than control(P<0.05 ).The contents of chlorophyll a,b and carotinoid in the leaves under 1 00%PbL stress decreased 42%,46%,52%,respectively,compared with the control.The values of relative con-ductivity in aboveground and underground parts under all treatments were higher than that of control.The activities of SOD increased as the PbL concentration enhanced and the activities of POD of aboveground and underground parts under 1 00%PbL stress were significantly highest and 1 .2 and 1 .5 times,respec-tively,those of the control (P<0.05 ).The highest contents of proline in aboveground and underground parts were found under the treatments of 1 00% PbL and both significant difference compared with the control(P<0.05 ).The AsA contents of I.pseudacorus L.decreased gradually as the level of PbL in-creased.The results indicated that I.pseudacorus had certain physiological tolerance to PbL and could be used for the phytoremediation of Pb tailings leaching fluid and its contaminated environment.