CAJ | 학술논문

目的探讨热应激预处理(HSP)对缺血皮瓣组织的保护作用及其与皮瓣中热休克蛋白70(HSP70)和热休克因子1(HSF1)的关系。方法将96只雄性Sprague Dawley 大鼠随机分为缺血组与实验组(HSP+缺血组)。缺血组：皮瓣模型制备后,阻断皮瓣蒂部第1h、2h、4h、6h、8h、10h共6个时间点,每时间点后观测皮瓣存活面积、HSP70、HSF1的变化,并进行统计学分析；实验组( HSP+缺血组)：先予以加热预处理,之后与缺血组相同。结果单纯缺血组皮瓣成活率下降率较实验组(HSP+缺血组)明显,差异有显著性(P<0.05),热应激预处理各组皮瓣的HSP70、HSF1合成量与皮瓣存活率呈正相关(r=0.7577,P<0.01)。结论热应激预处理能够改善皮瓣组织的存活率,HSP70、HSF1在热应激预处理改善皮瓣存活率的保护作用中发挥着重要的作用。
목적탐토열응격예처리(HSP)대결혈피판조직적보호작용급기여피판중열휴극단백70(HSP70)화열휴극인자1(HSF1)적관계。방법장96지웅성Sprague Dawley 대서수궤분위결혈조여실험조(HSP+결혈조)。결혈조：피판모형제비후,조단피판체부제1h、2h、4h、6h、8h、10h공6개시간점,매시간점후관측피판존활면적、HSP70、HSF1적변화,병진행통계학분석；실험조( HSP+결혈조)：선여이가열예처리,지후여결혈조상동。결과단순결혈조피판성활솔하강솔교실험조(HSP+결혈조)명현,차이유현저성(P<0.05),열응격예처리각조피판적HSP70、HSF1합성량여피판존활솔정정상관(r=0.7577,P<0.01)。결론열응격예처리능구개선피판조직적존활솔,HSP70、HSF1재열응격예처리개선피판존활솔적보호작용중발휘착중요적작용。
Objective This study was to reveal the continuous changes of HSP70 and HSF1 in the delayed and pro-tection of heat stress pretreatment on ischemic preconditioning axial flap in rats. Methods Animals were divided into two groups randomly:HSP+IPC group,ischemic pretreatment group( IPC) . In IPC group,rats skin flaps received ischemic pretreatment for 1 h,2 h,4 h,6 h,8 h,10 h,and the survival rate of the skin flaps,the contents of HSP70 and HSF1 were examined at the 6 time points. HSP+IPC group was given heat stress pretreatment and then the same treatment as IPC group. Results The survival rates of IPC descends more significantly than that of HSR+IPC. The survival rates of skin flaps have positive correlation with the contents of HSP70 and HSF1(r=0. 7577,P<0. 01). Conclusions Heat stress pretreatment can improve the survival rate of ischemia flap and the mechanism of the protection can be related to the func-tion of HSP70 and HSF1.