CAJ | 학술논문

在适宜温度（28±2）℃和低温（16±2）℃条件下，比较研究重复利用Zarrouk培养基（RZ）和新配制培养基（NZ）对钝顶螺旋藻（Spirulina platensis，SP）增殖和色素成分的影响，探讨培养基再利用对SP生长和低温耐受性的调节作用。结果发现，与采用N Z培养基相比，尽管在适宜温度下完全RZ培养基导致藻细胞增殖速率和生物量降低，但添加50％的RZ培养基（1/2 RZ ）不仅对藻细胞的增殖速率和生物量无明显影响，而且藻细胞中的主要色素含量明显升高。进一步发现，RZ可增强低温培养藻细胞对可见光的吸收，促进细胞叶绿素的含量。结果提示，培养基的合理再利用可获得SP最大生物量，增加SP中色素蛋白和胡萝卜素的累积，增强SP对低温的耐受性，因此对提高低温培养SP产量和品质具有一定的实践意义。
재괄의온도（28±2）℃화저온（16±2）℃조건하，비교연구중복이용Zarrouk배양기（RZ）화신배제배양기（NZ）대둔정라선조（Spirulina platensis，SP）증식화색소성분적영향，탐토배양기재이용대SP생장화저온내수성적조절작용。결과발현，여채용N Z배양기상비，진관재괄의온도하완전RZ배양기도치조세포증식속솔화생물량강저，단첨가50％적RZ배양기（1/2 RZ ）불부대조세포적증식속솔화생물량무명현영향，이차조세포중적주요색소함량명현승고。진일보발현，RZ가증강저온배양조세포대가견광적흡수，촉진세포협록소적함량。결과제시，배양기적합리재이용가획득SP최대생물량，증가SP중색소단백화호라복소적루적，증강SP대저온적내수성，인차대제고저온배양SP산량화품질구유일정적실천의의。
Spirulina platensis (SP)grows well in regions having higher temperature from 25 to 35 de-grees,but cold stress induced by lower temperature than 1 7 degree is a key environmental factor that leads to impaired growth of SP.Herein,effects of reused culture medium on SP growth and cell tolerance to low temperature stress were investigated.SP was cultivated in new or reused Zarrouk medium (named NZ or RZ)under ideal and low temperature (28 ±2 ℃ and 1 6 ±2 ℃,respectively),and comparative assay of cell growth,biomass,pigments,and visible spectrum were performed.Data showed that cultures with 50%of RZ added in NZ (named 1 /2RZ)under 28 ±2 ℃caused significant increases in concentra-tions of chlorophyll a (Chla),phycocyanin (PC)and carotenoids (Caro)pigments in SP cells,and also can amount to the maximum growth rate and biomass in compare with those of totally NZ control cultures. Furthermore,data showed that cultures with RZ under 1 6 ±2 ℃ induced increasing absorption peaks of visible spectrum of SP cells,which was in consistent with the accumulation of Chla content in SP cells. These findings implied the potential practice significance for reasonable application of reused culture me-dium in SP cultivation that can get a maximum biomass,promote the quality of SP product,and enhance the SP cell tolerance to low temperature.