CAJ | 학술논문

目的探讨人血清对生殖支原体( Mg)脂质相关膜蛋白( LAMPs)诱导TNF-α表达的影响。方法提取对数期Mg的LAMPs,分别加入脂蛋白脂肪酶,蛋白酶K及人血清预处理后,刺激THP-1细胞,ELISA法测定其TNF-α水平。结果 Mg LAMPs为一混合蛋白,其活性成分为脂质成分,能激活THP-1细胞表达大量TNF-α,且与LAMPs浓度呈明显的剂量依赖性。人血清能上调LAMPs诱导的TNF-α水平,且与LAMPs浓度呈明显的剂量依赖性。结论人血清上调Mg LAMPs诱导THP-1细胞表达TNF-α。
목적탐토인혈청대생식지원체( Mg)지질상관막단백( LAMPs)유도TNF-α표체적영향。방법제취대수기Mg적LAMPs,분별가입지단백지방매,단백매K급인혈청예처리후,자격THP-1세포,ELISA법측정기TNF-α수평。결과 Mg LAMPs위일혼합단백,기활성성분위지질성분,능격활THP-1세포표체대량TNF-α,차여LAMPs농도정명현적제량의뢰성。인혈청능상조LAMPs유도적TNF-α수평,차여LAMPs농도정명현적제량의뢰성。결론인혈청상조Mg LAMPs유도THP-1세포표체TNF-α。
Objective To investigate the effects of human serum on TNF-α expression activated by lipid-associated membrane proteins ( LAMPs) of Mycoplasma genitalium( Mg) . Methods LAMPs were extracted and pretreated with lipo-protein lipase or protease K or serum human. THP-1 cells were stimulated and the expression of TNF-α was detected by ELISA. Results LAMPs are mixed proteins and the active ingredients are lipid moiety. The secretion of TNF-αwas media-ted in LAMPs induced THP-1 cells and was significantly increased by LAMPs in a dose-dependent manner. TNF-αlevels were significantly up-regulated in LAMPs activated THP-1 cells by Human serum and was significantly increased by LAMPs in a dose-dependent manner. Conclusions The human serum raised TNF-αlevels by Mg LAMPs induced THP-1 cells.