中国油脂
中國油脂
중국유지
CHINA OILS AND FATS
2014年
5期
85-87
,共3页
免疫亲和柱净化%高效液相色谱-三重串联四级杆质谱法%黄曲霉毒素B1%食用植物油
免疫親和柱淨化%高效液相色譜-三重串聯四級桿質譜法%黃麯黴毒素B1%食用植物油
면역친화주정화%고효액상색보-삼중천련사급간질보법%황곡매독소B1%식용식물유
immunoaffinity column purification%HPLC-MS/MS%aflatoxin B1%edible vegetable oil
建立了免疫亲和柱净化-高效液相色谱-三重串联四级杆质谱法测定食用植物油中黄曲霉毒素B1的方法。采用70%甲醇水溶液提取食用植物油中黄曲霉毒素B1,提取液经过滤、免疫亲和柱净化后,上高效液相色谱-三重串联四级杆质谱仪进行测定。结果表明,黄曲霉毒素B1在0.2~10 ng/mL范围内方程线性关系良好,方法检出限(S/N=3)为0.05滋g/kg,定量限(S/N=10)为0.2滋g/kg,平均加标回收率在83.0%~94.8%之间,相对标准偏差小于7.8%。
建立瞭免疫親和柱淨化-高效液相色譜-三重串聯四級桿質譜法測定食用植物油中黃麯黴毒素B1的方法。採用70%甲醇水溶液提取食用植物油中黃麯黴毒素B1,提取液經過濾、免疫親和柱淨化後,上高效液相色譜-三重串聯四級桿質譜儀進行測定。結果錶明,黃麯黴毒素B1在0.2~10 ng/mL範圍內方程線性關繫良好,方法檢齣限(S/N=3)為0.05滋g/kg,定量限(S/N=10)為0.2滋g/kg,平均加標迴收率在83.0%~94.8%之間,相對標準偏差小于7.8%。
건립료면역친화주정화-고효액상색보-삼중천련사급간질보법측정식용식물유중황곡매독소B1적방법。채용70%갑순수용액제취식용식물유중황곡매독소B1,제취액경과려、면역친화주정화후,상고효액상색보-삼중천련사급간질보의진행측정。결과표명,황곡매독소B1재0.2~10 ng/mL범위내방정선성관계량호,방법검출한(S/N=3)위0.05자g/kg,정량한(S/N=10)위0.2자g/kg,평균가표회수솔재83.0%~94.8%지간,상대표준편차소우7.8%。
Immunoaffinity column purification and HPLC-MS/MS method was established to determine aflatoxin B1 in edible vegetable oil. Aflatoxin B1 was extracted from edible vegetable oil by 70% of meth-anol,then the extract was filtered and purified by immunoaffinity column before being injected into HPLC-MS/MS to determine aflatoxin B1 . The results showed that calibration curve presented good line-ar relationship when the mass concentration of aflatoxin B1 ranged from 0. 2 ng/mL to 10 ng/mL;the limit of detection(S/N=3) and the limit of quantification(S/N=10) of the method were 0. 05 μg/kg and 0. 2 μg/kg respectively,the average recovery of standard addition was in the range of 83. 0% -94. 8%, and the relative standard deviation was less than 7 . 8%.
