CAJ | 학술논문

采用两步法培养栅藻,即在低光强-含氮条件下,培养栅藻至稳定期积累生物量,然后将栅藻转移至高光强-缺氮培养基中积累油脂。结果表明：在低光强-含氮培养基BG11(+N)条件下,适宜栅藻生长的低光强为2000~2400 lx,培养期为10 d；转高光强-缺氮培养基BG11(-N)条件下培养,适宜栅藻积累油脂的高光强为6000~6500 lx,培养期为6 d。利用两步法培养栅藻,其生物量可达(0.957依0.126) g/L,油脂含量可达(23.35依0.2)%。两步法培养栅藻的生物量和油脂含量分别比常规单步法培养(10 d)的提高了35.74%和近1倍。
채용량보법배양책조,즉재저광강-함담조건하,배양책조지은정기적루생물량,연후장책조전이지고광강-결담배양기중적루유지。결과표명：재저광강-함담배양기BG11(+N)조건하,괄의책조생장적저광강위2000~2400 lx,배양기위10 d；전고광강-결담배양기BG11(-N)조건하배양,괄의책조적루유지적고광강위6000~6500 lx,배양기위6 d。이용량보법배양책조,기생물량가체(0.957의0.126) g/L,유지함량가체(23.35의0.2)%。량보법배양책조적생물량화유지함량분별비상규단보법배양(10 d)적제고료35.74%화근1배。
Scenedesmus sp. was cultured to stationary phase under the conditions of low illumination in-tensity with nitrogen,and then cultured in medium with high illumination intensity and nitrogen deficiency to accumulate oil. The results showed that in nitrogenous BG11 medium with low illumination intensity, the optimal illumination intensity for the growth of Scenedesmus sp. was 2 000-2 400 lx,culturing for 10 d; in nitrogen deficiency BG11 medium with high illumination intensity, the suitable illumination intensi-ty for oil accumulation of Scenedesmus sp. was 6 000-6 500 lx,culturing for 6 d. The biomass and oil con-tent of Scenedesmus sp. cultured by two-step method were (0. 957±0. 126) g/L and (23. 35±0. 2)% re-spectively, increasing by 35. 74% and 100% than that cultured by common one-step method.