CAJ | 학술논문

背景：Galectin-3属于半乳凝素家族成员，参与细胞生长和凋亡、细胞黏附、新生血管形成、肿瘤浸润和转移等多种生理和病理过程，在多种恶性肿瘤细胞中呈高表达。目的：研究 siRNA 干扰 galectin-3表达对人胃癌细胞株SGC-7901增殖、凋亡和化疗敏感性的影响。方法：合成靶向 galectin-3的 siRNA 并转染 SGC-7901细胞，以 real time PCR 和蛋白质印迹法检测干扰效果。CCK-8实验检测细胞增殖，流式细胞术检测细胞凋亡。结果：Galectin-3 siRNA 转染24 h 后转染效率为83．8％，转染后 SGC-7901细胞的 galectin-3表达显著受抑，mRNA 和蛋白表达量分别较空白对照组降低87．8％和90．4％（P ＜0．01）。转染后24 h、48 h 和72 h，galectin-3 siRNA 组 SGC-7901细胞增殖抑制率分别为15．57％±1．45％、32．90％±0．76％和57．35％±1．05％，转染后72 h 该组细胞凋亡率为46．17％±2．39％，均显著高于同时间点空白对照组、空脂质体组和阴性对照 siRNA 组（P ＜0．01）。Galectin-3 siRNA 组SGC-7901细胞由化疗药物奥沙利铂诱导的增殖抑制亦较其余三组显著增加（P ＜0．01）。结论：以 siRNA 干扰galectin-3表达后，SGC-7901细胞增殖减少、凋亡增加，对化疗药物的敏感性增强，表明 galectin-3有望成为胃癌基因治疗的有效靶点。
배경：Galectin-3속우반유응소가족성원，삼여세포생장화조망、세포점부、신생혈관형성、종류침윤화전이등다충생리화병리과정，재다충악성종류세포중정고표체。목적：연구 siRNA 간우 galectin-3표체대인위암세포주SGC-7901증식、조망화화료민감성적영향。방법：합성파향 galectin-3적 siRNA 병전염 SGC-7901세포，이 real time PCR 화단백질인적법검측간우효과。CCK-8실험검측세포증식，류식세포술검측세포조망。결과：Galectin-3 siRNA 전염24 h 후전염효솔위83．8％，전염후 SGC-7901세포적 galectin-3표체현저수억，mRNA 화단백표체량분별교공백대조조강저87．8％화90．4％（P ＜0．01）。전염후24 h、48 h 화72 h，galectin-3 siRNA 조 SGC-7901세포증식억제솔분별위15．57％±1．45％、32．90％±0．76％화57．35％±1．05％，전염후72 h 해조세포조망솔위46．17％±2．39％，균현저고우동시간점공백대조조、공지질체조화음성대조 siRNA 조（P ＜0．01）。Galectin-3 siRNA 조SGC-7901세포유화료약물오사리박유도적증식억제역교기여삼조현저증가（P ＜0．01）。결론：이 siRNA 간우galectin-3표체후，SGC-7901세포증식감소、조망증가，대화료약물적민감성증강，표명 galectin-3유망성위위암기인치료적유효파점。
Background:Galectin-3 is a member of the galectin family that participates in a variety of physiological and pathological events including cell growth and apoptosis,cell adhesion,angiogenesis,as well as tumor invasion and metastasis,and has been reported to be overexpressed in many human cancers.Aims:To investigate the effect of galactin-3 targeted RNA interference on proliferation,apoptosis and chemosensitivity of human gastric cancer cell line SGC-7901. Methods:Galectin-3 targeted siRNA was constructed and transfected into SGC-7901 cells.Efficacy of RNA interference was evaluated by real time PCR and Western blotting,while cell proliferation was assessed by CCK-8 assay and cell apoptosis by flow cytometry.Results:The transfection efficiency at 24 hours after transfection was 83.8%;expression of galectin-3 in SGC-7901 cells was significantly inhibited at mRNA and protein levels with a decreasing of 87.8% and 90.4%,respectively (P <0.01).Proliferation inhibition rates of SGC-7901 cells in galectin-3 siRNA group at 24,48 and 72 hours after transfection were 15.57% ±1.45%,32.90% ±0.76% and 57.35% ±1.05%,respectively,and the apoptosis rate at 72 hours after transfection was 46.17% ±2.39%;all were significantly higher than those in blank control,liposome control and negative siRNA control groups at the same time points (P <0.01).Proliferation inhibition of SGC-7901 cells induced by oxaliplatin,a chemotherapeutic agent,was also markedly increased in galectin-3 siRNA group (P <0.01).Conclusions:Expression of galectin-3 in SGC-7901 cells can be inhibited successfully by RNA interference;cell proliferation is decreased,cell apoptosis is increased and sensitivity to chemotherapeutic agent is augmented,which indicates that galectin-3 is a promising target for gastric cancer gene therapy.