农业科技与装备
農業科技與裝備
농업과기여장비
AGRICULTURAL SCIENCE & TECHNOLOGY AND EQUIPMENT
2014年
5期
17-18
,共2页
姜力耘%邵屹%王战男%郭铁丰%王博
薑力耘%邵屹%王戰男%郭鐵豐%王博
강력운%소흘%왕전남%곽철봉%왕박
DNA提取%银杏叶片%RAPD%PCR
DNA提取%銀杏葉片%RAPD%PCR
DNA제취%은행협편%RAPD%PCR
DNA extraction%gingko%RAPD%PCR
在CTAB法的基础上，建立适于银杏叶片DNA微量快速提取的新方法。经紫外分光光度和琼脂糖凝胶电泳检测，该方法提取的DNA在质量和产量上都可满足后续扩增反应的要求。与常规CTAB方法相比，该方法操作简单、速度快、时间短。
在CTAB法的基礎上，建立適于銀杏葉片DNA微量快速提取的新方法。經紫外分光光度和瓊脂糖凝膠電泳檢測，該方法提取的DNA在質量和產量上都可滿足後續擴增反應的要求。與常規CTAB方法相比，該方法操作簡單、速度快、時間短。
재CTAB법적기출상，건립괄우은행협편DNA미량쾌속제취적신방법。경자외분광광도화경지당응효전영검측，해방법제취적DNA재질량화산량상도가만족후속확증반응적요구。여상규CTAB방법상비，해방법조작간단、속도쾌、시간단。
A method of rapid DNA extraction from gingko was established based on CTAB procedure. Utraviolet spectrophotometer and agarose gel electrophoresis methods were used to test the DNA quality. The DNA extracted with this method can meet the requirement for the follow-up amplified reaction both in terms of quality and quantity. Compared with CTAB procedure, this method is simpler, faster and more costs-saving.
