CAJ | 학술논문

采用多菌混合发酵可以提高纤维素酶的活力，为获得高活力的纤维素酶制剂，文中以碱处理后的甘蔗渣和麸皮作为发酵产酶培养基，采用响应面法对2株纤维素酶生产菌里氏木霉CICC40359和斜卧青霉SMX固态混合发酵条件进行了优化。结果发现在发酵温度为28℃，料水比（质量体积比）1∶2．5（g/mL）的条件下，当V（青霉）∶V（木霉）为3∶1，总接种量8％（mL/g），培养基中 m（蔗渣）∶m（麸皮）为2∶1，发酵3 d 时，滤纸酶活有最大值达到101．825 FPU/g，这为后续优化工作的开展提供了依据，同时高酶活下发酵液中呈现高的糖质量分数为同步产酶发酵产乙醇提供了新思路。
채용다균혼합발효가이제고섬유소매적활력，위획득고활력적섬유소매제제，문중이감처리후적감자사화부피작위발효산매배양기，채용향응면법대2주섬유소매생산균리씨목매CICC40359화사와청매SMX고태혼합발효조건진행료우화。결과발현재발효온도위28℃，료수비（질량체적비）1∶2．5（g/mL）적조건하，당V（청매）∶V（목매）위3∶1，총접충량8％（mL/g），배양기중 m（자사）∶m（부피）위2∶1，발효3 d 시，려지매활유최대치체도101．825 FPU/g，저위후속우화공작적개전제공료의거，동시고매활하발효액중정현고적당질량분수위동보산매발효산을순제공료신사로。
Cellulase production efficiency can be improved via the mixed culture solid-state fermentation.In order to obtain high cellulase activity,alkali-treated sugarcane bagasse supplemented with wheat bran was used as enzyme fermentation substrate.The response surface method was introduced to optimize the solid-state mixed-fermentation with Trichoderma reesei CICC40359 and Penicillium decumbens SMX.The results indicate that the filter paper enzyme activity has an maximum value of 101.825 FPU/g under the following conditions:temperature is 28 ℃;mass and volume ratio of material to water is 1 ∶2.5 g/mL;V(Penicillium )∶V(Trichoderma) =3 ∶1, m(bagasse)∶m(wheat bran)=2∶1 ,inoculum dose is 0.08 g/mL and fermentation time is 3 d,which provides a basis for the next optimization work.The fermentation liquor showed high sugar content when the enzyme activity was high.These results indicate that simultaneous enzyme and ethanol production may be an effective approach for the economic production of bioethanol.