CAJ | 학술논문

采用RT-PCR方法扩增猪的FST基因完整编码区，采用双酶切和连接方法构建可在肌肉细胞中特异表达的真核载体，并转染C2C12细胞系进行验证。结果表明，猪FST基因全长1032 bp，与网上已提交序列相似度为99.13%，成功构建可在肌肉细胞中特异表达的真核载体pEGFP-C1-α-actin-FST，转染C2C12细胞后，通过实时定量PCR检测，表明FST基因过表达可抑制MSTN基因表达。
채용RT-PCR방법확증저적FST기인완정편마구，채용쌍매절화련접방법구건가재기육세포중특이표체적진핵재체，병전염C2C12세포계진행험증。결과표명，저FST기인전장1032 bp，여망상이제교서렬상사도위99.13%，성공구건가재기육세포중특이표체적진핵재체pEGFP-C1-α-actin-FST，전염C2C12세포후，통과실시정량PCR검측，표명FST기인과표체가억제MSTN기인표체。
RT-PCR was used to amplify the entire coding region of the FST gene pigs, With double enzymes and the connection method to build can be specific in the muscle cells of eukaryotic expression vector, and transfected C2C12 cells for verification. The results showed that pigs FST gene was 1 032 bp, with the online submitted was 99.13% sequence similarity, successfully constructed a specific expression in the muscle cells of the eukaryotic expression vector pEGFP-C1-α-actin-FST, transfected C2C12 cells, by Real-time quantitative PCR, showed overexpression FST could inhibit MSTN gene expression.