CAJ | 학술논문

对我国新见病害富贵草叶斑病的症状进行了描述，结合病原形态特征、培养特性及rDNA-ITS序列分析结果，确定该病害由Volutella pachysandricoal 引起。病菌的生物学特性研究结果表明：菌丝生长较佳培养基为PCA、V8汁和PDA，较佳产孢培养基为CMA和V8汁。菌丝生长适宜温度为15～28℃，最适温度为25℃；25℃时产孢量最高。菌丝在pH＝7～11时生长良好，pH＝7～8最适产孢。光照对菌丝生长无显著影响，但有利于病菌产孢。病菌孢子致死温度为52℃，10 min。用生长速率法测定病菌对20种杀菌剂敏感性的结果表明：病菌对50％肟菌·25％戊唑醇WG、25％丙环唑EC、50％多菌灵WP、40％氟硅唑 EC、77％氢氧化铜WP、30％噁霉灵AS的敏感性较高，其EC50值均小于1．0 mg/L，EC90值均小于10．0 mg/L。
대아국신견병해부귀초협반병적증상진행료묘술，결합병원형태특정、배양특성급rDNA-ITS서렬분석결과，학정해병해유Volutella pachysandricoal 인기。병균적생물학특성연구결과표명：균사생장교가배양기위PCA、V8즙화PDA，교가산포배양기위CMA화V8즙。균사생장괄의온도위15～28℃，최괄온도위25℃；25℃시산포량최고。균사재pH＝7～11시생장량호，pH＝7～8최괄산포。광조대균사생장무현저영향，단유리우병균산포。병균포자치사온도위52℃，10 min。용생장속솔법측정병균대20충살균제민감성적결과표명：병균대50％우균·25％무서순WG、25％병배서EC、50％다균령WP、40％불규서 EC、77％경양화동WP、30％오매령AS적민감성교고，기EC50치균소우1．0 mg/L，EC90치균소우10．0 mg/L。
The symptoms of Pachysandra Volutella blight were described .With the morphology , cultural characteristics of the i-solates and ITS sequence , the pathogen was identified as Volutella pachysandricola.The results of the biological character-istics of the pathogen show that the optimal media for mycelium growth are PCA, V8 juice and PDA, but CMA and V8 juice are the best for spore production .The suitable temperature for mycelium growth and spore production is 25℃.The suitable pH for mycelium growth is 7-11, and 7-8 is optimal for spore production.Light can promote spore production and has no effect on mycelium growth.The lethal temperature of conidia is 49℃in 10 min.The susceptibility of 20 fungicides to Volutella pachysandricola was detected by mycelium growth rate method.The pathogen is more sensitive to 50%triflox-ystrobin· 25%tebuconazole WG, 25%propiconazole EC, 50%carbendazim WP, 40%flusilazole EC, 77%copper hy-droxide WP, and 30%hymexazol AS with EC50<1.0 mg/L and EC90<100. mg/L.