国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
10期
1261-1262,1265
,共3页
曾慧%周万青%张之烽
曾慧%週萬青%張之烽
증혜%주만청%장지봉
鲍氏不动杆菌%喹诺酮类%抗药性 ,细菌%基因 ,gy rA%基因 ,p arC
鮑氏不動桿菌%喹諾酮類%抗藥性 ,細菌%基因 ,gy rA%基因 ,p arC
포씨불동간균%규낙동류%항약성 ,세균%기인 ,gy rA%기인 ,p arC
Acinetobacter baumannii%quinolones%drug resistance,bacterial%genes,gyrA%genes,parC
目的:探讨鲍氏不动杆菌对喹诺酮类药物耐药机制及菌株同源性分析。方法收集临床分离的喹诺酮类耐药鲍氏不动杆菌25株,采用纸片扩散法(K-B)检测其对常规药物的敏感性;采用聚合酶链反应(PCR)检测喹诺酮类耐药相关基因 gyrA和 parC基因,并经限制性内切酶酶切及测序方法验证;基因外重复回文序列(REP)-PCR分析菌株同源性。结果25株鲍氏不动杆菌对12种抗菌药物呈现出多重耐药,仅对米诺环素和阿米卡星敏感,敏感率分别为48.0%和32.0%,对多黏菌素B全部敏感[最低抑菌浓度(MIC)≤2μg/mL];所有菌株检出 gyrA和 parC基因,25株菌株均存在 gyrA基因第83位密码子TCA→TTA突变(Ser→Leu),23株菌株存在 parC基因第80位密码子TCG→TTG突变(Ser→Leu),2株菌株存在 parC基因第84位GAA→GGA突变(Glu→Gly );REP-PCR显示,所测菌株具有很高的同源性。结论耐喹诺酮类鲍氏不动杆菌具有很高的同源性,存在gy rA和 p arC基因突变位点。
目的:探討鮑氏不動桿菌對喹諾酮類藥物耐藥機製及菌株同源性分析。方法收集臨床分離的喹諾酮類耐藥鮑氏不動桿菌25株,採用紙片擴散法(K-B)檢測其對常規藥物的敏感性;採用聚閤酶鏈反應(PCR)檢測喹諾酮類耐藥相關基因 gyrA和 parC基因,併經限製性內切酶酶切及測序方法驗證;基因外重複迴文序列(REP)-PCR分析菌株同源性。結果25株鮑氏不動桿菌對12種抗菌藥物呈現齣多重耐藥,僅對米諾環素和阿米卡星敏感,敏感率分彆為48.0%和32.0%,對多黏菌素B全部敏感[最低抑菌濃度(MIC)≤2μg/mL];所有菌株檢齣 gyrA和 parC基因,25株菌株均存在 gyrA基因第83位密碼子TCA→TTA突變(Ser→Leu),23株菌株存在 parC基因第80位密碼子TCG→TTG突變(Ser→Leu),2株菌株存在 parC基因第84位GAA→GGA突變(Glu→Gly );REP-PCR顯示,所測菌株具有很高的同源性。結論耐喹諾酮類鮑氏不動桿菌具有很高的同源性,存在gy rA和 p arC基因突變位點。
목적:탐토포씨불동간균대규낙동류약물내약궤제급균주동원성분석。방법수집림상분리적규낙동류내약포씨불동간균25주,채용지편확산법(K-B)검측기대상규약물적민감성;채용취합매련반응(PCR)검측규낙동류내약상관기인 gyrA화 parC기인,병경한제성내절매매절급측서방법험증;기인외중복회문서렬(REP)-PCR분석균주동원성。결과25주포씨불동간균대12충항균약물정현출다중내약,부대미낙배소화아미잡성민감,민감솔분별위48.0%화32.0%,대다점균소B전부민감[최저억균농도(MIC)≤2μg/mL];소유균주검출 gyrA화 parC기인,25주균주균존재 gyrA기인제83위밀마자TCA→TTA돌변(Ser→Leu),23주균주존재 parC기인제80위밀마자TCG→TTG돌변(Ser→Leu),2주균주존재 parC기인제84위GAA→GGA돌변(Glu→Gly );REP-PCR현시,소측균주구유흔고적동원성。결론내규낙동류포씨불동간균구유흔고적동원성,존재gy rA화 p arC기인돌변위점。
Objective To explore the mechanisms of quinolones resistance in Acinetobacter baumannii and homology analysis a-mong the strains .Methods 25 strains of quinolones-resistant Acinetobacter baumannii isolated clinically were collected .Kirby-Bauer(K-B) detection was utilized to detect the sensitivity of conventional drugs ,and polymerase chain reaction (PCR) was em-ployed to detect quinolone resistance-related genes gyrA and parC which were verified by restriction enzyme digestion and sequen-cing ,repetitive extragenic palindrome(REP)-PCR was adopted to analyze the strain homology .Results Multiple resistances to 12 kinds of antibacterial agents were found among the 25 strains of Acinetobacter baumannii which were sensitive only to minocycline and amikacin ,with sensitive rates were 48 .0% and 32 .0% ,respectively ,and were all sensitive to polymyxin B [minimal inhibitory concentration(MIC)≤2 μg/mL] .gyrA and parC genes were found in the all strain .Mutation TCA→TTA(Ser→Leu) at coden 83 in gyrA gene existed in 25 strains ,mutation TCG→TTG(Ser→Leu) at coden 80 in parC gene existed in 23 strains ,mutation GAA→GGA(Glu→Gly) at coden 84 in parC gene existed in 2 strains .REP-PCR showed that the strains had high degree of homology . Conclusion Quinolone-resistant Acinetobacter baumannii has high degree of homology ,existing gyrA and parC gene mutations .