国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
10期
1245-1247
,共3页
多态性 ,单核苷酸%糖尿病 ,妊娠%易感基因
多態性 ,單覈苷痠%糖尿病 ,妊娠%易感基因
다태성 ,단핵감산%당뇨병 ,임신%역감기인
polymorphism,single nucleotide%diabetes,gestational%susceptibility genes
目的：探讨江苏地区2型糖尿病易感基因 CDKAL1、CDKN2A-CDKN2B、TCF7L2及 HHEX-IDE的单核苷酸多态性（SNP）与妊娠期糖尿病（GDM ）的关系。方法采用病例对照法及多重SNaPshot SNP分型技术检测185例无GDM 倾向的健康孕妇（对照组）及176例GDM孕妇（GDM 组）CDKAL1、CDKN2A-CDKN2B、TCF7L2及 HHEX-IDE的SNP位点基因分型，并对所有SNP位点进行连续不平衡分析。结果比较GDM 组和对照组孕妇的等位基因频率，CDKAL1（rs7754840）的等位基因C为GDM的一个危险因素。CC型携带者患 GDM 的风险是未携带者的1．73倍[ P＜0．01，OR＝1．73，95％ C I：1．28～2．33]。CDK N2A-CDK N2B（rs10811661）、TCF7L2（rs7903146）及 H HEX-IDE（rs1111875）检测位点的基因分布差异无统计学差异（P＞0．05）。 CDKAL1（rs7754840）、CDKN2A-CDKN2B（rs10811661）、TCF7L2（rs7903146）及 HHEX-IDE（rs1111875）的基因型频率均符合遗传平衡法则。结论 CDKAL1（rs7754840）与江苏地区GDM 易感密切相关。
目的：探討江囌地區2型糖尿病易感基因 CDKAL1、CDKN2A-CDKN2B、TCF7L2及 HHEX-IDE的單覈苷痠多態性（SNP）與妊娠期糖尿病（GDM ）的關繫。方法採用病例對照法及多重SNaPshot SNP分型技術檢測185例無GDM 傾嚮的健康孕婦（對照組）及176例GDM孕婦（GDM 組）CDKAL1、CDKN2A-CDKN2B、TCF7L2及 HHEX-IDE的SNP位點基因分型，併對所有SNP位點進行連續不平衡分析。結果比較GDM 組和對照組孕婦的等位基因頻率，CDKAL1（rs7754840）的等位基因C為GDM的一箇危險因素。CC型攜帶者患 GDM 的風險是未攜帶者的1．73倍[ P＜0．01，OR＝1．73，95％ C I：1．28～2．33]。CDK N2A-CDK N2B（rs10811661）、TCF7L2（rs7903146）及 H HEX-IDE（rs1111875）檢測位點的基因分佈差異無統計學差異（P＞0．05）。 CDKAL1（rs7754840）、CDKN2A-CDKN2B（rs10811661）、TCF7L2（rs7903146）及 HHEX-IDE（rs1111875）的基因型頻率均符閤遺傳平衡法則。結論 CDKAL1（rs7754840）與江囌地區GDM 易感密切相關。
목적：탐토강소지구2형당뇨병역감기인 CDKAL1、CDKN2A-CDKN2B、TCF7L2급 HHEX-IDE적단핵감산다태성（SNP）여임신기당뇨병（GDM ）적관계。방법채용병례대조법급다중SNaPshot SNP분형기술검측185례무GDM 경향적건강잉부（대조조）급176례GDM잉부（GDM 조）CDKAL1、CDKN2A-CDKN2B、TCF7L2급 HHEX-IDE적SNP위점기인분형，병대소유SNP위점진행련속불평형분석。결과비교GDM 조화대조조잉부적등위기인빈솔，CDKAL1（rs7754840）적등위기인C위GDM적일개위험인소。CC형휴대자환 GDM 적풍험시미휴대자적1．73배[ P＜0．01，OR＝1．73，95％ C I：1．28～2．33]。CDK N2A-CDK N2B（rs10811661）、TCF7L2（rs7903146）급 H HEX-IDE（rs1111875）검측위점적기인분포차이무통계학차이（P＞0．05）。 CDKAL1（rs7754840）、CDKN2A-CDKN2B（rs10811661）、TCF7L2（rs7903146）급 HHEX-IDE（rs1111875）적기인형빈솔균부합유전평형법칙。결론 CDKAL1（rs7754840）여강소지구GDM 역감밀절상관。
Objective To investigate the relationship between single nucleotide polymorphism (SNP) of susceptibility genes CD-KAL1 ,CDKN2A-CDKN2B ,TCF7L2 and HHEX-IDE of type 2 diabetes mellitus and gestational diabetes mellitus (GDM) in Jiangsu region .Methods 185 healthy pregnant women without GDM tendency (control group) and 176 pregnant women with GDM (GDM group) were enrolled .Case-control method and Multiplex SNaPshot SNP genotyping technology were utilized to detect genotypes at SNP loci of CDKAL1 ,CDKN2A-CDKN2B ,TCF7L2 and HHEX-IDE of pregnant women both in the two groups , and all SNP loci were subject to continuous disequilibrium analysis .Results Compared the allele frequencies of pregnant women in GDM group and the control group ,C allele of CDKAL1(rs7754840) was a risk factor for GDM .The risk of suffering GDM of CC genotype carriers was 1 .73 times the non-CC genotype carriers[P<0 .01 ,OR=1 .73 ,95% CI:1 .28-2 .33] .No statistical difference wasfound in gene distribution of detection loci of CDKN2A-CDKN2B(rs10811661),TCF7L2(rs7903146) and HHEX-IDE (rs1111875) .The genotype frequencies of CDKAL1(rs7754840) ,CDKN2A-CDKN2B(rs10811661) ,TCF7L2(rs7903146) and H HEX-IDE(rs1111875) were all accordance with the law of genetic equilibrium .Conclusion CDKAL1(rs7754840) is closely re-lated to GDM susceptibility in Jiangsu region .
