CAJ | 학술논문

目的：探讨自发性高血压大鼠（SHR）脑组织胱硫醚β合酶（CBS）的表达及分布情况。方法选择SD（Sprague Daw-ley）大鼠和SHR各8只，采用逆转录聚合酶链反应（RT-PCR）和real time-PCR技术检测其大脑皮层、小脑、延髓、下丘脑、海马组织CBS mRNA的表达。结果 RT-PCR检测，SHR下丘脑、海马、小脑、大脑皮层及延髓组织的 CBS mRNA表达水平分别为0．93±0．01、0．09±0．03、0．36±0．06、0．07±0．01及0．39±0．02，显著低于SD大鼠（P＜0．05）。real-time PCR检测，SD大鼠脑组织 CBS mRNA的表达水平从高到低依次为大脑皮层、海马、延髓、下丘脑、小脑。SHR脑组织 CBS mRNA的表达从高到低依次为下丘脑、延髓、大脑皮层、海马及小脑。SHR脑组织 CBS mRNA的表达水平低于SD大鼠（P＜0．05）。结论 SHR脑组织中CBS mRNA的表达下调，可能通过中枢机制参与高血压的发病。
목적：탐토자발성고혈압대서（SHR）뇌조직광류미β합매（CBS）적표체급분포정황。방법선택SD（Sprague Daw-ley）대서화SHR각8지，채용역전록취합매련반응（RT-PCR）화real time-PCR기술검측기대뇌피층、소뇌、연수、하구뇌、해마조직CBS mRNA적표체。결과 RT-PCR검측，SHR하구뇌、해마、소뇌、대뇌피층급연수조직적 CBS mRNA표체수평분별위0．93±0．01、0．09±0．03、0．36±0．06、0．07±0．01급0．39±0．02，현저저우SD대서（P＜0．05）。real-time PCR검측，SD대서뇌조직 CBS mRNA적표체수평종고도저의차위대뇌피층、해마、연수、하구뇌、소뇌。SHR뇌조직 CBS mRNA적표체종고도저의차위하구뇌、연수、대뇌피층、해마급소뇌。SHR뇌조직 CBS mRNA적표체수평저우SD대서（P＜0．05）。결론 SHR뇌조직중CBS mRNA적표체하조，가능통과중추궤제삼여고혈압적발병。
Objective To investigate the cystathionine beta-synthase(CBS) expression and distribution in brain tissue of sponta-neous hypertensive rats(SHR) .Methods Eight SD(Sprague Dawley) rats and eight SHR were enrolled .Reverse transcriptase-pol-ymerase chain reaction(RT-PCR) and real time-PCR techniques were adopted to detect the CBS mRNA expression in their cerebral cortex ,cerebellum ,medulla oblongata ,under thalamus ,hippocampus tissue .Results According to RT-PCR ,CBS mRNA expression levels in hypothalamus ,hippocampus ,cerebellum ,cerebral cortex and medulla tissue of SHR were 0 .93 ± 0 .01 ,0 .09 ± 0 .03 ,0 .36 ± 0 .06 ,0 .07 ± 0 .01 and 0 .39 ± 0 .02 ,respectively ,which were significantly lower than those of SD rats (P<0 .05) .According to real-time PCR ,CBS mRNA expression levels in brain tissue of SD rats from high to low in order were cerebral cortex ,hippocampus ,me-dulla oblongata ,hypothalamus and cerebellum .Those of SHR from high to low in order were hypothalamus ,medulla ,cortex ,hippo-campus and cerebellum .CBS mRNA expression levels in brain tissue of SHR were lower than those of SD rats (P<0 .05) .Conclu-sion The expression level of CBS mRNA in brain tissue of SHR is down-regulated ,which may be involved in the pathogenesis of hypertension via central mechanism .