CAJ | 학술논문

目的：探讨白色念珠菌蛋白指纹库的建立，为白色念珠菌感染快速诊断奠定基础。方法收集96株临床分离的白色念珠菌，提取DNA ，采用聚合酶链反应（PCR）扩增其ITS1-5．8S-ITS2基因片段，利用限制性内切酶对其进行鉴定。应用表面增强激光解析电离飞行时间质谱（SELDI-TOF-MS）仪检测念珠菌蛋白指纹，Ciphergen ProteinChip软件自动采集数据，筛选稳定表达蛋白峰建立白色念珠菌蛋白指纹库，运用经准确鉴定的念珠菌对建立的蛋白指纹库进行验证。结果限制性片段长度多态性分析证实临床分离的所有菌株均为白色念珠菌。SELDI-TOF-MS芯片能捕获15个蛋白峰，其中5个蛋白峰在所有白色念珠菌中均稳定表达，利用相似性分析软件建立白色念珠菌蛋白指纹库，白色念珠菌蛋白指纹与建立数据库的相似性大于95％，而其他种类念珠菌蛋白指纹与数据库的相似性均小于50％。结论白色念珠菌蛋白指纹库的建立为快速诊断白色念珠菌感染提供了理论依据。
목적：탐토백색념주균단백지문고적건립，위백색념주균감염쾌속진단전정기출。방법수집96주림상분리적백색념주균，제취DNA ，채용취합매련반응（PCR）확증기ITS1-5．8S-ITS2기인편단，이용한제성내절매대기진행감정。응용표면증강격광해석전리비행시간질보（SELDI-TOF-MS）의검측념주균단백지문，Ciphergen ProteinChip연건자동채집수거，사선은정표체단백봉건립백색념주균단백지문고，운용경준학감정적념주균대건립적단백지문고진행험증。결과한제성편단장도다태성분석증실림상분리적소유균주균위백색념주균。SELDI-TOF-MS심편능포획15개단백봉，기중5개단백봉재소유백색념주균중균은정표체，이용상사성분석연건건립백색념주균단백지문고，백색념주균단백지문여건립수거고적상사성대우95％，이기타충류념주균단백지문여수거고적상사성균소우50％。결론백색념주균단백지문고적건립위쾌속진단백색념주균감염제공료이론의거。
Objective To explore the establishment of peptide mapping database of Candida albicans ,laying the foundation for rapid diagnosis of Candida albicans infection .Methods 96 Candida albicans were collected clinically ,and its DNA was extracted . Polymerase chain reaction(PCR) was used to amplify the ITS1-5 .8S-ITS2 gene fragments and restriction endonucleases were a-dopted to identify them .Surface enhanced laser desorption ionization-time of flight-mass spectrometry(SELDI-TOF-MS) instrument was applied to detect the Candida albicans peptide mapping ,and Ciphergen ProteinChip software was used to collect data automati-cally .The established peptide mapping database was verified by confirmed Candida .Results According to restriction fragment length polymorphism analysis ,96 strains were confirmed as Candida albicans .15 peptide peaks were captured by SELDI-TOF-MS chips .Five peptide peaks of them with stable expression were screened out ,and the similarity analysis software was used to estab-lish peptide mapping database of Candida albicans .More than 95% of similarity was found between peptide mapping of Candida albicans and established database ,while less than 50% was found between peptide mapping of other Candida species and database . Conclusion The establishment of peptide mapping database of Candida albicans provides a theoretical basis for the rapid diagnosis of Candida albicans infection .