CAJ | 학술논문

目的：探讨产肺炎克雷伯菌碳青霉烯酶（K PC ）铜绿假单胞菌的耐药性。方法铜绿假单胞菌菌株来自2例痰液标本。采用VITEK 2 COMPACT全自动微生物鉴定/药敏分析仪进行细菌菌株的鉴定，应用聚合酶链反应（PCR）及基因测序法鉴定K PC酶基因型，采用肉汤稀释法进行抗菌药的最低抑菌浓度（M IC ）测定。结果2例菌株均对β-内酰胺类抗菌药、左氧氟沙星耐药，对环丙沙星中介，对庆大霉素、奈替米星、妥布霉素、黏菌素及多黏菌素B敏感；其中1株还对替加环素敏感。2例菌株所产碳青霉烯酶不是金属β-内酰胺酶，其亚型为 KPC-2。接合实验未能证明 bla K PC 基因能接合转移到大肠埃希菌 J53中。结论产K PC铜绿假单胞菌的出现给临床抗感染治疗带来严峻挑战。
목적：탐토산폐염극뢰백균탄청매희매（K PC ）동록가단포균적내약성。방법동록가단포균균주래자2례담액표본。채용VITEK 2 COMPACT전자동미생물감정/약민분석의진행세균균주적감정，응용취합매련반응（PCR）급기인측서법감정K PC매기인형，채용육탕희석법진행항균약적최저억균농도（M IC ）측정。결과2례균주균대β-내선알류항균약、좌양불사성내약，대배병사성중개，대경대매소、내체미성、타포매소、점균소급다점균소B민감；기중1주환대체가배소민감。2례균주소산탄청매희매불시금속β-내선알매，기아형위 KPC-2。접합실험미능증명 bla K PC 기인능접합전이도대장애희균 J53중。결론산K PC동록가단포균적출현급림상항감염치료대래엄준도전。
Objective To investigate the drug-resistance of Pseudomonas aeruginosa which producing K lebsiella pneumoniae carbapenemases(KPC) .Methods Pseudomonas aeruginosa strains were derived from two sputum samples .VITEK 2 COMPACT Automated Microbial Identification/Susceptibility Analyzer was employed to identify the bacterial strains .Polymerase chain reaction (PCR) and gene sequencing were adopted to identify the genotypes of KPC enzyme ,Broth dilution method was used to measure the minimal inhibitory concentration(MIC) of antimicrobial agents .Results Both Pseudomonas aeruginosa strains were resistant to β-lactam antibiotic and levofloxacin ,and were intermediary to ciprofloxacin ,and sensitive to gentamicin ,netilmicin ,tobramycin ,colistin and multi-polymyxin B .One of them was sensitive to tigecycline .Carbapenemase produced by the two stains was not metal β-lacta-mase ,with the subtype of KPC-2 .Mating experiments failed to prove the bla K PC gene could be transferred to E .coli J53 .Conclu-sion Appearance of KPC-producing Pseudomonas aeruginosa poses serious challenges to clinical anti-infective therapy .