CAJ | 학술논문

目的：探讨重组人胱抑素C（Cys C）及其多克隆抗体的制备。方法根据大肠埃希菌编码蛋白的特性设计Cys C编码基因序列，人工合成目的基因，并将其在大肠埃希菌中表达，目的蛋白经亲和层析纯化后免疫新西兰大白兔；采用Western blot和酶联免疫吸附测定（ELISA）法检测兔血清重组人Cys C及其多克隆抗体。结果制备的重组人Cys C纯度达90％以上，抗重组人Cys C抗体具有很好的结合效价和特异性。结论制备的重组人Cys C及其抗体可用于进一步的实验研究和临床应用。
목적：탐토중조인광억소C（Cys C）급기다극륭항체적제비。방법근거대장애희균편마단백적특성설계Cys C편마기인서렬，인공합성목적기인，병장기재대장애희균중표체，목적단백경친화층석순화후면역신서란대백토；채용Western blot화매련면역흡부측정（ELISA）법검측토혈청중조인Cys C급기다극륭항체。결과제비적중조인Cys C순도체90％이상，항중조인Cys C항체구유흔호적결합효개화특이성。결론제비적중조인Cys C급기항체가용우진일보적실험연구화림상응용。
Objective To explore the preparation of recombinant human cystatin C (Cys C ) and its polyclonal antibody . Methods The gene sequences encoding Cys C were designed according to characteristics of E .coli protein-coding ,and the target gene was artificially synthesized and expressed in E .coli .The target protein was purified by affinity chromatography and was used to immunize the New Zealand white rabbits .Western blot and enzyme-linked immunosorbent assay(ELISA) were adopted to detect the recombinant human Cys C and its polyclonal antibodies in the rabbits serum .Results Purity of prepared recombinant human Cys C was more than 90% .Anti-recombinant human Cys C antibody showed good binding activities and specificity .Conclusion Prepared recombinant human Cys C and its antibody can be used in further experimental research and clinical application .