CAJ | 학술논문

目的：探讨自身抗体临界值质控血清的制备并对其进行评价。方法将献血员血清置于56℃30 min灭活，离心去除沉淀后，按1∶2、1∶4、1∶6稀释比例检测试剂盒中多批号的阳性对照质控物。结果抗 RNP/Sm抗体（＋＋＋）：1∶2稀释后，显色程度均为＋＋＋；1∶4、1∶6稀释后，显色程度均为＋＋。抗SS-A抗体（＋＋～＋＋＋）：1∶2稀释后，显色程度均为＋＋；1∶4、1∶6稀释后，显色程度为＋～＋＋。抗RO-52抗体（＋＋～＋＋＋）：1∶2稀释后，显色为＋～＋＋；1∶4、1∶6稀释后，显色程度为＋。抗核小体抗体：1∶1、1∶2稀释后可出现阳性；1∶4、1∶6稀释后，显色均为阴性。结论自身抗体质控血清制备方便，质量可靠，可满足临床检测室内质控需要。
목적：탐토자신항체림계치질공혈청적제비병대기진행평개。방법장헌혈원혈청치우56℃30 min멸활，리심거제침정후，안1∶2、1∶4、1∶6희석비례검측시제합중다비호적양성대조질공물。결과항 RNP/Sm항체（＋＋＋）：1∶2희석후，현색정도균위＋＋＋；1∶4、1∶6희석후，현색정도균위＋＋。항SS-A항체（＋＋～＋＋＋）：1∶2희석후，현색정도균위＋＋；1∶4、1∶6희석후，현색정도위＋～＋＋。항RO-52항체（＋＋～＋＋＋）：1∶2희석후，현색위＋～＋＋；1∶4、1∶6희석후，현색정도위＋。항핵소체항체：1∶1、1∶2희석후가출현양성；1∶4、1∶6희석후，현색균위음성。결론자신항체질공혈청제비방편，질량가고，가만족림상검측실내질공수요。
Objective To investigate and evaluate the preparation of quality control serum of autoantibodies threshold . Methods Donors′ serum blood were placed in 56 ℃ for 30 min to inactivate ,followed by centrifuged to remove the precipitates ,and then multi-batch quality control material in detection kit were detected in dilution ratios of 1∶2 ,1∶4 ,1∶6 .Results Anti-RNP/Sm antibody(+ + + ):after 1∶2 dilution ,the coloration degree was + + + ;after 1∶4 ,1∶6 dilution ,the coloration degree was ++ .Anti-SS-A antibody(+ + to + + + ):after 1∶2 dilution ,the coloration degree was + + ;after 1∶4 ,1∶6 dilution ,the colora-tion degree was + to + + .Anti-RO-52 antibody(+ + to + + + ):after 1∶2 dilution ,the coloration degree was + to + + ;after 1∶4 ,1∶6 dilution ,the coloration degree was + .Anti-nucleosome antibodies :positive were found in dilution of 1∶1 ,1∶2;after 1∶4 ,1∶6 dilution ,the coloration was negative .Conclusion Preparation of quality control serum of autoantibodies is convenient , reliable ,and can meet the needs of clinical detection of internal quality control .