CAJ | 학술논문

目的：探讨罕见豚鼠耳炎奴卡菌的鉴定方法并评价其药物敏感性。方法采用形态、生理生化表型鉴定与16S rRNA序列分析相结合的方法鉴定菌株，使用微量肉汤法检测其药物敏感性。结果临床分离的奴卡菌被鉴定为豚鼠耳炎奴卡菌，其对哌拉西林、红霉素、头孢他啶、头孢哌酮、庆大霉素、头孢西丁、哌拉西林/他唑巴坦耐药，万古霉素、左氧氟沙星、亚胺培南、依替沙星、环丙沙星、阿米卡星、复方磺胺甲基异噁唑敏感。结论16S rRNA基因序列分析法可用以鉴定豚鼠耳炎奴卡菌，磺胺类药物治疗有效。
목적：탐토한견돈서이염노잡균적감정방법병평개기약물민감성。방법채용형태、생리생화표형감정여16S rRNA서렬분석상결합적방법감정균주，사용미량육탕법검측기약물민감성。결과림상분리적노잡균피감정위돈서이염노잡균，기대고랍서림、홍매소、두포타정、두포고동、경대매소、두포서정、고랍서림/타서파탄내약，만고매소、좌양불사성、아알배남、의체사성、배병사성、아미잡성、복방광알갑기이오서민감。결론16S rRNA기인서렬분석법가용이감정돈서이염노잡균，광알류약물치료유효。
Objective To study the identification of rare Nocardiaotitidiscaviarum and its drug sensitivity .Methods Morpho-logical ,physiological and biochemical phenotype identification methods combined with 16S rRNA sequence analysis were employed to identified the bacteria strains .Broth method was used to detect their drug sensitivity .Results The clinically isolated Nocardia strain was identified as Nocardiaotitidiscaviarum which was resistant to piperacillin ,erythromycin ,ceftazidime ,cefoperazone ,gen-tamicin ,cefoxitin ,piperacillin/tazobactam ,and was sensitive to vancomycin ,levofloxacin ,imipenem ,etimicin ,ciprofloxacin ,amika-cin ,sulfamethoxazole .Conclusion 16S rRNA gene sequence analysis can be used to identify Nocardia otitidiscaviarum ,and sulfa drugs shows good therapeutic effect .