CAJ | 학술논문

目的：评价噬菌体生物扩增技术（PhaB ）在肺结核诊断中的应用。方法回顾性分析采用PhaB法、3D培养法、直接涂片法检测373份痰标本的抗酸杆菌结果。结果 PhaB法、3D培养法、直接涂片法检测抗酸杆菌的阳性率分别为53．6％、56．6％及20．1％。以3D培养法结果为标准，直接涂片法的敏感性、特异度、准确性分别为33．2％、95．7％及60．3％；PhaB法的敏感性、特异度、准确性分别为89．1％、93．6％及90．6％。直接涂片法与PhaB法检测结果比较，差异有统计学意义（χ2＝94．7，P＜0．01）；PhaB法与3D培养法检测结果比较，差异无统计学意义（χ2＝3．46，P＞0．05）。结论 PhaB法检测结核分枝杆菌具有较高的敏感性、特异度、准确性，值得在临床推广。
목적：평개서균체생물확증기술（PhaB ）재폐결핵진단중적응용。방법회고성분석채용PhaB법、3D배양법、직접도편법검측373빈담표본적항산간균결과。결과 PhaB법、3D배양법、직접도편법검측항산간균적양성솔분별위53．6％、56．6％급20．1％。이3D배양법결과위표준，직접도편법적민감성、특이도、준학성분별위33．2％、95．7％급60．3％；PhaB법적민감성、특이도、준학성분별위89．1％、93．6％급90．6％。직접도편법여PhaB법검측결과비교，차이유통계학의의（χ2＝94．7，P＜0．01）；PhaB법여3D배양법검측결과비교，차이무통계학의의（χ2＝3．46，P＞0．05）。결론 PhaB법검측결핵분지간균구유교고적민감성、특이도、준학성，치득재림상추엄。
Objective To evaluate application of phage amplified biologically assay (PhaB) in the diagnosis of pulmonary tuber-culosis .Methods The results of acid-fast bacillus in 373 sputum specimens obtained by PhaB method ,3D culture and direct smear assay were retrospectively analyzed .Results The positive rates of acid-fast bacillus detected by PhaB method ,3D culture and the direct smear assay were 53 .6% ,56 .6% and 20 .1% ,respectively .Set the 3D culture result as the standard ,the sensitivity ,specifici-ty and accuracy of the direct smear assay were 33 .2% ,95 .7% and 60 .3% ,respectively ,and those of PhaB method were 89 .1% , 93 .6% and 90 .6% ,respectively .Compared the direct smear assay with PhaB method ,the difference showed statistical significance (χ2 =94 .7 ,P<0 .01) .Compared PhaB method with 3D culture ,the difference showed no statistical significance (χ2 =3 .46 ,P>0 .05) .Conclusion PhaB method has high sensitivity ,specificity and accuracy in Mycobacterium tuberculosis detection and is wor-thy of promotion .