CAJ | 학술논문

目的：分析药疹患者外周血淋巴细胞亚群的变化。方法将18例初诊药疹患者作为药疹组，根据致敏药物的不同将其分为头孢菌素组（n＝9）、青霉素组（n＝5）及中药组（n＝4），将20例健康体检者作为对照组。应用流式细胞仪检测其外周血T淋巴细胞（CD3＋、CD3＋CD4＋、CD3＋CD8＋）、B淋巴细胞、自然杀伤细胞（NK）和自然杀伤 T 淋巴细胞（NKT）所占百分比及其绝对计数。结果药疹组患者外周血T淋巴细胞（CD3＋、CD3＋ CD4＋）、B淋巴细胞和N K T细胞所占百分比与对照组的差异有统计学意义（P＜0．05）；药疹组患者CD3＋CD8＋淋巴细胞所占百分比与对照组的差异无统计学意义（P＞0．05），而其 T、B淋巴细胞亚群的绝对计数与对照组的差异有统计学意义（P＜0．05）。结论药疹患者外周血CD3＋、CD3＋CD4＋淋巴细胞所占百分比降低，NKT细胞所占百分比增高，这可能与患者免疫调节有关。
목적：분석약진환자외주혈림파세포아군적변화。방법장18례초진약진환자작위약진조，근거치민약물적불동장기분위두포균소조（n＝9）、청매소조（n＝5）급중약조（n＝4），장20례건강체검자작위대조조。응용류식세포의검측기외주혈T림파세포（CD3＋、CD3＋CD4＋、CD3＋CD8＋）、B림파세포、자연살상세포（NK）화자연살상 T 림파세포（NKT）소점백분비급기절대계수。결과약진조환자외주혈T림파세포（CD3＋、CD3＋ CD4＋）、B림파세포화N K T세포소점백분비여대조조적차이유통계학의의（P＜0．05）；약진조환자CD3＋CD8＋림파세포소점백분비여대조조적차이무통계학의의（P＞0．05），이기 T、B림파세포아군적절대계수여대조조적차이유통계학의의（P＜0．05）。결론약진환자외주혈CD3＋、CD3＋CD4＋림파세포소점백분비강저，NKT세포소점백분비증고，저가능여환자면역조절유관。
Objective To analyze the changes of lymphocyte subsets in peripheral blood of patients with drug eruption . Methods 18 newly diagnosed patients were served as the drug eruption group ,and were subdivided into cephalosporin group (n=9) ,penicillin group(n=5) and Chinese medicine group(n=4) according to different sensitizing drugs .20 healthy people were taken as the control group .Flow cytometry were utilized to detect the percentages and absolute counts of T lymphocytes (CD3+ ,CD3+CD4+ and CD3+CD8+ ) ,B lymphocytes ,natural killer cell(NK) and natural killer T lymphocytes(NKT) in their peripheral blood . Results Differences of percentages of T lymphocytes (CD3+ ,CD3+ CD4+ ) ,B lymphocytes ,NKT cells between the drug eruption group and the control group showed statistical significant (P<0 .05) .Difference of percentages of CD3+ CD8+ lymphocytes of pa-tients between the drug eruption group and the control group demonstrated no statistical significant (P>0 .05) ,while that of abso-lute counts of T and B lymphocytes of patients was statistical significant between the drug eruption group and the control group (P<0 .05) .Conclusion The percentages of CD3+ ,CD3+CD4+ lymphocytes of patients with drug eruption decrease ,while those of NKT cells increase ,which may be related to the patients′immune regulation .