中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
19期
3036-3041
,共6页
张毓文%张晓庆%周宁%刘兴元
張毓文%張曉慶%週寧%劉興元
장육문%장효경%주저%류흥원
干细胞%脐带脐血干细胞%蛛网膜下腔阻滞%新生儿%脐血%单个核细胞%神经细胞%星形胶质细胞
榦細胞%臍帶臍血榦細胞%蛛網膜下腔阻滯%新生兒%臍血%單箇覈細胞%神經細胞%星形膠質細胞
간세포%제대제혈간세포%주망막하강조체%신생인%제혈%단개핵세포%신경세포%성형효질세포
subarachnoid space%anesthesia,obstetrical%fetal blood%glial fibrilary acidic protein%intermediate filament proteins
背景：有学者认为麻醉状态可提高脐血采集分离质量，目前尚无蛛网膜下腔阻滞对脐血单个核细胞向神经干细胞及星形胶质细胞分化影响的研究。<br> 目的：观察蛛网膜下腔阻滞对新生儿脐血单个核细胞向神经干细胞及星形胶质细胞方向分化的影响。<br> 方法：选择顺产新生儿及蛛网膜下腔阻滞剖宫产新生儿脐血标本各20份，分别列为对照组及研究组，在胎儿娩出后抽取脐血分离接种脐血单个核细胞，并进行体外培养，每份标本常规培养3 d后分别进行非诱导分化培养和向神经细胞方向诱导分化培养。对培养前后细胞进行神经干细胞、星形胶质细胞标志性Nestin、GFAP抗原免疫组化鉴定。<br> 结果与结论：蛛网膜下腔阻滞剖宫产新生儿脐血单个核细胞诱导分化培养后表达 Nestin、GFAP 抗原阳性细胞，与顺产新生儿比较差异无显著性意义(P >0.05)。结果显示蛛网膜下腔阻滞对新生儿脐血单个核细胞向神经干细胞及星形胶质细胞方向分化没有影响。
揹景：有學者認為痳醉狀態可提高臍血採集分離質量，目前尚無蛛網膜下腔阻滯對臍血單箇覈細胞嚮神經榦細胞及星形膠質細胞分化影響的研究。<br> 目的：觀察蛛網膜下腔阻滯對新生兒臍血單箇覈細胞嚮神經榦細胞及星形膠質細胞方嚮分化的影響。<br> 方法：選擇順產新生兒及蛛網膜下腔阻滯剖宮產新生兒臍血標本各20份，分彆列為對照組及研究組，在胎兒娩齣後抽取臍血分離接種臍血單箇覈細胞，併進行體外培養，每份標本常規培養3 d後分彆進行非誘導分化培養和嚮神經細胞方嚮誘導分化培養。對培養前後細胞進行神經榦細胞、星形膠質細胞標誌性Nestin、GFAP抗原免疫組化鑒定。<br> 結果與結論：蛛網膜下腔阻滯剖宮產新生兒臍血單箇覈細胞誘導分化培養後錶達 Nestin、GFAP 抗原暘性細胞，與順產新生兒比較差異無顯著性意義(P >0.05)。結果顯示蛛網膜下腔阻滯對新生兒臍血單箇覈細胞嚮神經榦細胞及星形膠質細胞方嚮分化沒有影響。
배경：유학자인위마취상태가제고제혈채집분리질량，목전상무주망막하강조체대제혈단개핵세포향신경간세포급성형효질세포분화영향적연구。<br> 목적：관찰주망막하강조체대신생인제혈단개핵세포향신경간세포급성형효질세포방향분화적영향。<br> 방법：선택순산신생인급주망막하강조체부궁산신생인제혈표본각20빈，분별렬위대조조급연구조，재태인면출후추취제혈분리접충제혈단개핵세포，병진행체외배양，매빈표본상규배양3 d후분별진행비유도분화배양화향신경세포방향유도분화배양。대배양전후세포진행신경간세포、성형효질세포표지성Nestin、GFAP항원면역조화감정。<br> 결과여결론：주망막하강조체부궁산신생인제혈단개핵세포유도분화배양후표체 Nestin、GFAP 항원양성세포，여순산신생인비교차이무현저성의의(P >0.05)。결과현시주망막하강조체대신생인제혈단개핵세포향신경간세포급성형효질세포방향분화몰유영향。
BACKGROUND:Some scholars believe that anesthesia can improve the quality of cord blood separation and colection, and subarachnoid block is commonly used in cesarean section, but there is no research on the influence of subarachnoid block on differentiation potential of mononuclear cels into neural stem cels and astrocytes. <br> OBJECTIVE: To study the influence of subarachnoid block on differentiation potential of mononuclear cels in the cord blood of neonates into neural stem cels and astrocytes. <br> METHODS:Mononuclear cels isolated from 20 neonates delivered spontaneously and 20 neonates born by cesarean delivery undergoing subarachnoid block were culturedin vitro, acting as control group and study group. After delivery, cord blood samples were taken to isolate and culture mononuclear celsin vitro. After 3 days of routine culture, the cels were subject to non-induced culture and induced differentiation into neural stem cels. The cultured cels were identified by the cellmakers Nestin and glial fibrilary acidic protein with immunohistochemistry identification. <br> RESULTS AND CONCLUSION:The Nestin and glial fibrilary acidic protein showed no difference between the two groups after induced differentiation (P > 0.05), indicating subarachnoid block has no impact on the differentiation of mononuclear cels in the cord blood from neonates into neural stem cels and astrocytes.
