中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
19期
3054-3059
,共6页
张育森%鲍世韵%张悦%李富荣
張育森%鮑世韻%張悅%李富榮
장육삼%포세운%장열%리부영
干细胞%移植%胎鼠%胰腺干细胞%胰岛%胰岛移植%联合移植%糖尿病
榦細胞%移植%胎鼠%胰腺榦細胞%胰島%胰島移植%聯閤移植%糖尿病
간세포%이식%태서%이선간세포%이도%이도이식%연합이식%당뇨병
islets of langerhans%islets of langerhans transplantation%pancreas%insulin
背景:胰腺干细胞可在体外维持胰岛的结构,减少胰岛细胞坏死及凋亡,延长胰岛的体外存活时间,保护胰岛的活性。<br> 目的:探索胎鼠胰腺干细胞与胰岛共移植体内保护移植胰岛,提高胰岛移植疗效的可行性。<br> 方法:将成年大鼠35只随机等分为联合移植组、单独胰岛移植组、单纯胰腺干细胞移植组、模型组及对照组,前4组均腹腔注射链脲佐菌素-柠檬酸盐缓冲液建立糖尿病模型。联合移植组、单独胰岛移植组、单纯胰腺干细胞大鼠分别在左侧肾包膜下移植分离纯化孕16 d SD大鼠胎鼠胰腺干细胞和/或成年SD大鼠胰岛。<br> 结果与结论:联合移植组大鼠移植后5 d内血糖可降至正常,血浆胰岛素达到正常水平,胰岛存活时间(18.2±2.4) d;单独移植组大鼠血糖可于移植后1周内降至正常,胰岛存活时间(14.4±2.1) d;两组胰岛存活时间比较差异有显著性意义(P <0.05)。而其他组糖尿病大鼠血糖均未能降至正常范围。说明胎鼠胰腺干细胞与胰岛共移植可延长胰岛体内存活时间,保护胰岛功能,提高移植疗效。
揹景:胰腺榦細胞可在體外維持胰島的結構,減少胰島細胞壞死及凋亡,延長胰島的體外存活時間,保護胰島的活性。<br> 目的:探索胎鼠胰腺榦細胞與胰島共移植體內保護移植胰島,提高胰島移植療效的可行性。<br> 方法:將成年大鼠35隻隨機等分為聯閤移植組、單獨胰島移植組、單純胰腺榦細胞移植組、模型組及對照組,前4組均腹腔註射鏈脲佐菌素-檸檬痠鹽緩遲液建立糖尿病模型。聯閤移植組、單獨胰島移植組、單純胰腺榦細胞大鼠分彆在左側腎包膜下移植分離純化孕16 d SD大鼠胎鼠胰腺榦細胞和/或成年SD大鼠胰島。<br> 結果與結論:聯閤移植組大鼠移植後5 d內血糖可降至正常,血漿胰島素達到正常水平,胰島存活時間(18.2±2.4) d;單獨移植組大鼠血糖可于移植後1週內降至正常,胰島存活時間(14.4±2.1) d;兩組胰島存活時間比較差異有顯著性意義(P <0.05)。而其他組糖尿病大鼠血糖均未能降至正常範圍。說明胎鼠胰腺榦細胞與胰島共移植可延長胰島體內存活時間,保護胰島功能,提高移植療效。
배경:이선간세포가재체외유지이도적결구,감소이도세포배사급조망,연장이도적체외존활시간,보호이도적활성。<br> 목적:탐색태서이선간세포여이도공이식체내보호이식이도,제고이도이식료효적가행성。<br> 방법:장성년대서35지수궤등분위연합이식조、단독이도이식조、단순이선간세포이식조、모형조급대조조,전4조균복강주사련뇨좌균소-저몽산염완충액건립당뇨병모형。연합이식조、단독이도이식조、단순이선간세포대서분별재좌측신포막하이식분리순화잉16 d SD대서태서이선간세포화/혹성년SD대서이도。<br> 결과여결론:연합이식조대서이식후5 d내혈당가강지정상,혈장이도소체도정상수평,이도존활시간(18.2±2.4) d;단독이식조대서혈당가우이식후1주내강지정상,이도존활시간(14.4±2.1) d;량조이도존활시간비교차이유현저성의의(P <0.05)。이기타조당뇨병대서혈당균미능강지정상범위。설명태서이선간세포여이도공이식가연장이도체내존활시간,보호이도공능,제고이식료효。
BACKGROUND:Pancreatic stem cels can maintain the islet structurein vitro, reduce necrosis and apoptosis of islet cels,in vitro prolong islet survival, and keep islet activity. <br> OBJECTIVE: To observe the possibility of preserving viability of isletsin vivo by co-transplanting fetal rat pancreatic stem cels and islets so as to improve the outcome of islet transplantation. <br> METHODS:Thirty-five adult rats were randomly divided into five groups, including co-transplantation, islet transplantation alone, pancreatic stem celltransplantation alone, diabetes control and normal control groups. Diabetic models were established in the former four groups by intraperitoneal injection of streptozotocin-citrate buffer. Pancreatic stem cels from the fetal rats at pregnant 16 days or islets from adult Sprague-Dawley rats. RESULTS AND CONCLUSION:In the co-transplantation group, the levels of blood glucose and plasma insulin returned to the normal after 5 days of co-transplantation, and the survival time of islets was (18.2±2.4) days. In the islet transplantation alone group, the level of blood glucose was reduced to normal after 1 week of transplantation, and the survival time of islets was (14.4±2.1) days. Significant different was found between the survival time of islets between this two groups (P < 0.05). However, the level of blood glucose was stil abnormal in the other groups. These findings indicate that the co-transplantation of fetal rat pancreatic stem cels and islets can prolong the survival time of isletsin vivo, protect viability of islets and improve the outcome of islet transplantation.