CAJ | 학술논문

目的检测半乳糖凝集素l(Galectin-1)在胃癌组织、癌旁组织、正常胃组织的表达,探讨其与胃癌发生、发展、侵袭转移及预后的关系.方法 (1)用链霉菌抗生物素蛋白-过氧化物酶(SP)免疫组织化学法检测124例临床组织标本(包括正常胃组织124例,癌旁组织124例,胃癌组织124例)中的Galectin-1蛋白的表达.(2)用逆转录-聚合酶链反应(RT-PCR)法及Western blot分析Galectin-1在124对胃癌组织、相应癌旁组织及正常胃组织中的表达.(3)用采用Kaplan-Meier法绘制患者的生存曲线,Log-rank法分析患者生存情况.结果胃癌组织中Galectin-1的表达水平(特别是低分化胃癌组织中Galectin-1的表达)显著高于癌旁组织及正常胃组织(P＜0.01).Galectin-1阳性的患者术后生存时间普遍低于Galectin-1阴性的患者.Galectin-1阳性的胃癌患者术后复发率及转移率明显高于Galectin-1阴性的患者.结论 Galectin-1在胃癌的侵袭转移过程中起着重要的作用,与胃癌淋巴结转移及患者术后预后密切相关.
목적 검측반유당응집소l(Galectin-1)재위암조직、암방조직、정상위조직적표체,탐토기여위암발생、발전、침습전이급예후적관계.방법 (1)용련매균항생물소단백-과양화물매(SP)면역조직화학법검측124례림상조직표본(포괄정상위조직124례,암방조직124례,위암조직124례)중적Galectin-1단백적표체.(2)용역전록-취합매련반응(RT-PCR)법급Western blot분석Galectin-1재124대위암조직、상응암방조직급정상위조직중적표체.(3)용채용Kaplan-Meier법회제환자적생존곡선,Log-rank법분석환자생존정황.결과 위암조직중Galectin-1적표체수평(특별시저분화위암조직중Galectin-1적표체)현저고우암방조직급정상위조직(P＜0.01).Galectin-1양성적환자술후생존시간보편저우Galectin-1음성적환자.Galectin-1양성적위암환자술후복발솔급전이솔명현고우Galectin-1음성적환자.결론 Galectin-1재위암적침습전이과정중기착중요적작용,여위암림파결전이급환자술후예후밀절상관.
Objective To investigate the expression of Galectin-1 in gastric cancer and their clinical significanc.Methods Streptavid-in-peroxidase (SP) immunohistochemistry was used to detect the expression of galectin-1 in 124 specimens including 124 normal tissue,124 carcinoma sides tissues,124 gastric carcinomaand.Real-time RT-PCR and Western blotting was used to detect galectin-1 expression in 124 fresh specimens of gastric carcinoma and normal gastric tissue.The survival curve was drawn by Kaplan-Meier method,and the survival rates of the patients were analyzed by Log-rank test.Results The Galectin-1 expression in cancerous tissue increased significantly compared with that in the para-cancerous tissue (P ＜ 0.01),the positive expression of galectin-1 has more recurTence and shorter survival.Conclusion Galectin-1 may be involved in the development and progression of gastric cancer,and relate to the infiltration,differentiation,lymphnode metastasis and recurrence of gastric cancer.