中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
5期
1120-1122,封4
,共4页
杜超%成超%卢晓明%舒晓刚%刘俊
杜超%成超%盧曉明%舒曉剛%劉俊
두초%성초%로효명%서효강%류준
AT富集序列特异性结合蛋白1%直肠癌%转移%预后
AT富集序列特異性結閤蛋白1%直腸癌%轉移%預後
AT부집서렬특이성결합단백1%직장암%전이%예후
Special AT-rich sequence binding protein 1%Rectal cancer%Metastasis%Prognosis
目的 探讨AT富集序列特异性结合蛋白1(SATB1)在直肠癌组织中的表达及其临床意义.方法 分别采用实时定量聚合酶链反应(Real-time PCR)和免疫组织化学法检测直肠癌组织中SATB1 mRNA与蛋白水平的表达,并分析两者相关性;应用统计学方法分析SATB1表达与患者临床病理参数之间的关系及其预后意义.结果 直肠癌及正常对照组织中SATB1 mRNA相对表达量分别为1.93±0.36和0.75±0.19,蛋白阳性表达率分别为42.5％和6.25％,直肠癌组织均显著高于正常组织(P＜0.05);SATB1 mRNA与其蛋白表达水平呈显著正相关(r=0.649;P＜0.05).SATB1高表达与直肠癌患者较高的TNM分期、淋巴结转移及远处转移之间相关(P＜0.05).结论 SATB1在直肠癌组织中的表达水平显著上调;SATB1高表达与直肠癌的侵袭、转移潜能密切相关.
目的 探討AT富集序列特異性結閤蛋白1(SATB1)在直腸癌組織中的錶達及其臨床意義.方法 分彆採用實時定量聚閤酶鏈反應(Real-time PCR)和免疫組織化學法檢測直腸癌組織中SATB1 mRNA與蛋白水平的錶達,併分析兩者相關性;應用統計學方法分析SATB1錶達與患者臨床病理參數之間的關繫及其預後意義.結果 直腸癌及正常對照組織中SATB1 mRNA相對錶達量分彆為1.93±0.36和0.75±0.19,蛋白暘性錶達率分彆為42.5％和6.25％,直腸癌組織均顯著高于正常組織(P＜0.05);SATB1 mRNA與其蛋白錶達水平呈顯著正相關(r=0.649;P＜0.05).SATB1高錶達與直腸癌患者較高的TNM分期、淋巴結轉移及遠處轉移之間相關(P＜0.05).結論 SATB1在直腸癌組織中的錶達水平顯著上調;SATB1高錶達與直腸癌的侵襲、轉移潛能密切相關.
목적 탐토AT부집서렬특이성결합단백1(SATB1)재직장암조직중적표체급기림상의의.방법 분별채용실시정량취합매련반응(Real-time PCR)화면역조직화학법검측직장암조직중SATB1 mRNA여단백수평적표체,병분석량자상관성;응용통계학방법분석SATB1표체여환자림상병리삼수지간적관계급기예후의의.결과 직장암급정상대조조직중SATB1 mRNA상대표체량분별위1.93±0.36화0.75±0.19,단백양성표체솔분별위42.5％화6.25％,직장암조직균현저고우정상조직(P＜0.05);SATB1 mRNA여기단백표체수평정현저정상관(r=0.649;P＜0.05).SATB1고표체여직장암환자교고적TNM분기、림파결전이급원처전이지간상관(P＜0.05).결론 SATB1재직장암조직중적표체수평현저상조;SATB1고표체여직장암적침습、전이잠능밀절상관.
Objective To investigate special AT-rich sequence binding protein 1 (SATB1) ex-pression and its clinical significance in the rectal cancer tissues.Methods The expression of SATB1 mRNA and protein was detected by real-time quantitative polymerase chain reaction (Real-time PCR) and immunohistochemical staining respectively in 80 cases of rectal cancer.The correlations between SATB1 expression and clinicopathological factors and its prognostic significance were statistically analyzed.Results The relative levels of SATB1 mRNA in rectal cancer tissues and normal tissues were 1.93 ± 0.36 and 0.75 ±0.19,respectively.The positive rate of SATB1 protein in cancer and control tissues was 42.5％and 6.25％,respectively.Both SATB1 mRNA and protein expression was significantly up-regulated in rectal cancer specimens as compared with that in normal tissues (P ＜0.05 for both),and the SATB1 mRNA expression agreed well with the immunohistochemical findings (r =0.649 ; P ＜ 0.05).Moreover,SATB1 expression was positively correlated with higher TNM stage,lymph node metastasis and distant metastasis (P ＜ 0.05 for each).Conclusion Over-expression of SATB1 could be associated with the developmental and metastatic potential of human rectal cancer.
