CAJ | 학술논문

目的研究地塞米松治疗放射性脑水肿与erb-B1基因转录水平的关系. 方法60只大鼠建立脑胶质瘤模型后按照随机数字表法分为3组:A组(种植C6细胞15 d后给予Co<'60>照射并于照射前后给予地塞米松治疗)、B组(种植C6细胞15 d后仅给予Co<'60>照射)、C组(种植C6细胞后既不给予照射也不给予地塞米松治疗),各组均为20只.所有大鼠种植C6细胞16 d后将大鼠处死,测量大鼠脑水肿情况和erb-B1转录水平. 结果种植C6细胞21 d后3组大鼠脑水肿情况:B组脑水肿情况最严重,其次为A组、C组,任两组比较差异均有统计学意义(P<0.05).erb-B1转录水平:B组表达水平最高,其次为A组、C组,任两组比较差异均有统计学意义(P<0.05). 结论地塞米松可以有效防治放射性脑水肿.地塞米松可以通过抑制erb-B1基因转录水平来治疗放射性脑水肿.
목적 연구지새미송치료방사성뇌수종여erb-B1기인전록수평적관계. 방법60지대서건립뇌효질류모형후안조수궤수자표법분위3조:A조(충식C6세포15 d후급여Co<'60>조사병우조사전후급여지새미송치료)、B조(충식C6세포15 d후부급여Co<'60>조사)、C조(충식C6세포후기불급여조사야불급여지새미송치료),각조균위20지.소유대서충식C6세포16 d후장대서처사,측량대서뇌수종정황화erb-B1전록수평. 결과충식C6세포21 d후3조대서뇌수종정황:B조뇌수종정황최엄중,기차위A조、C조,임량조비교차이균유통계학의의(P<0.05).erb-B1전록수평:B조표체수평최고,기차위A조、C조,임량조비교차이균유통계학의의(P<0.05). 결론지새미송가이유효방치방사성뇌수종.지새미송가이통과억제erb-B1기인전록수평래치료방사성뇌수종.
Objective To investigate the relation between the transcription level of erb-B1 gene and the therapeutic effect of dexamethasone on radiation-induced brain edema in rats. Methods Sixty rats bearing implanted C6 cell glioma in the brain were randomized into groups A, B, and C (n=20). Fifteen days after the intracerebral cell implantation, the rats in group A received <'60>Co exposure with dexamethasone treatment before and alter the exposure, and those in group B had the exposure only. The rats in group C received no exposure or dcxamethasone treatment after the cell implantation. Six days after <'60>Co exposure, the rats were sacrificed to evaluate the brain edema and detect the expression level of erb-B1 mRNA in the brain tissue. Results Twenty-one days after C6 cell implantation, the rats in group B showed the most serious cerebral edema, followed by those in group A and group C, with significant difference between any of the two groups (P<0.05). The expression level oferb-B1 mRNA differed significantly between the 3 groups, which was the highest in group B followed by group A and group C (P<0.05). Conclusion Dexamethasone can effectively prevent and ameliorate radiation-induced brain edema probably by suppressing the transcription level oferb-B1 gene.