中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
20期
3218-3222
,共5页
刘亮%王磊%童亚林%莫永亮%吕璐%陈云鹏%杨文贤%吕礼芳%詹球%朱富军%辛海明%龚震宇
劉亮%王磊%童亞林%莫永亮%呂璐%陳雲鵬%楊文賢%呂禮芳%詹毬%硃富軍%辛海明%龔震宇
류량%왕뢰%동아림%막영량%려로%진운붕%양문현%려례방%첨구%주부군%신해명%공진우
组织构建%组织工程%人羊膜匀浆上清液%许旺细胞%细胞增殖%细胞活力%周围神经%细胞生长曲线%MTT法%国家自然科学基金
組織構建%組織工程%人羊膜勻漿上清液%許旺細胞%細胞增殖%細胞活力%週圍神經%細胞生長麯線%MTT法%國傢自然科學基金
조직구건%조직공정%인양막균장상청액%허왕세포%세포증식%세포활력%주위신경%세포생장곡선%MTT법%국가자연과학기금
amnion%Schwann cells%cellproliferation%cytokines
背景:许旺细胞是周围神经修复过程的重要细胞,而研究发现人羊膜细胞分泌的多种细胞因子能够促进许旺细胞增殖。<br> 目的:观察不同浓度人羊膜匀浆上清液对鼠许旺细胞(RSC96)生长的影响。<br> 方法:使用含体积分数20%胎牛血清的高糖DMEM培养基原代培养RSC96细胞株,传代至第2代用于实验研究。根据人羊膜匀浆上清液在培养基中的不同体积分数(0,10,15,20,25%)分组。<br> 结果与结论:人羊膜匀浆上清液的总蛋白浓度为675 mg/L,表皮生长因子、碱性成纤维生长因子和血管内皮生长因子浓度分别为(470.625±2.546),(4.121±0.026)和(0.172±0.002) ng/L。在培养第1-7天,10%和15%人羊膜匀浆上清液组的增殖率大于20%和25%人羊膜匀浆上清液组(P <0.05);10%、15%人羊膜匀浆上清液组显示出促进细胞增殖的作用,而20%、25%人羊膜匀浆上清液组显示出抑制细胞增殖的作用;各实验组的细胞活力与对照组接近(P >0.05)。提示人羊膜匀浆上清液低浓度时(10%和15%)具有促进RSC96增殖作用,高浓度时(20%和25%)抑制RSC96增殖。
揹景:許旺細胞是週圍神經脩複過程的重要細胞,而研究髮現人羊膜細胞分泌的多種細胞因子能夠促進許旺細胞增殖。<br> 目的:觀察不同濃度人羊膜勻漿上清液對鼠許旺細胞(RSC96)生長的影響。<br> 方法:使用含體積分數20%胎牛血清的高糖DMEM培養基原代培養RSC96細胞株,傳代至第2代用于實驗研究。根據人羊膜勻漿上清液在培養基中的不同體積分數(0,10,15,20,25%)分組。<br> 結果與結論:人羊膜勻漿上清液的總蛋白濃度為675 mg/L,錶皮生長因子、堿性成纖維生長因子和血管內皮生長因子濃度分彆為(470.625±2.546),(4.121±0.026)和(0.172±0.002) ng/L。在培養第1-7天,10%和15%人羊膜勻漿上清液組的增殖率大于20%和25%人羊膜勻漿上清液組(P <0.05);10%、15%人羊膜勻漿上清液組顯示齣促進細胞增殖的作用,而20%、25%人羊膜勻漿上清液組顯示齣抑製細胞增殖的作用;各實驗組的細胞活力與對照組接近(P >0.05)。提示人羊膜勻漿上清液低濃度時(10%和15%)具有促進RSC96增殖作用,高濃度時(20%和25%)抑製RSC96增殖。
배경:허왕세포시주위신경수복과정적중요세포,이연구발현인양막세포분비적다충세포인자능구촉진허왕세포증식。<br> 목적:관찰불동농도인양막균장상청액대서허왕세포(RSC96)생장적영향。<br> 방법:사용함체적분수20%태우혈청적고당DMEM배양기원대배양RSC96세포주,전대지제2대용우실험연구。근거인양막균장상청액재배양기중적불동체적분수(0,10,15,20,25%)분조。<br> 결과여결론:인양막균장상청액적총단백농도위675 mg/L,표피생장인자、감성성섬유생장인자화혈관내피생장인자농도분별위(470.625±2.546),(4.121±0.026)화(0.172±0.002) ng/L。재배양제1-7천,10%화15%인양막균장상청액조적증식솔대우20%화25%인양막균장상청액조(P <0.05);10%、15%인양막균장상청액조현시출촉진세포증식적작용,이20%、25%인양막균장상청액조현시출억제세포증식적작용;각실험조적세포활력여대조조접근(P >0.05)。제시인양막균장상청액저농도시(10%화15%)구유촉진RSC96증식작용,고농도시(20%화25%)억제RSC96증식。
BACKGROUND:Schwann cells are important celllines in the process of repairing peripheral nerve injury, and human amniotic homogenate supernatant is shown to secrete a variety of cytokines, which could promote the proliferation of Schwann cells. <br> OBJECTIVE:To investigate the effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cell96. <br> METHODS:Schwann cell96 was cultured with high-glucose DMEM containing 20%fetal bovine serum, and the second generation of Schwann cell96 was applied for experiments. The cultured cells were divided into five groups according to different volume fractions of human amniotic homogenate supernatant (0%, 10%, 15%, 20%, 25%) in the medium. <br> RESULTS AND CONCLUSION:The total protein concentration of human amniotic homogenate supernatant was 675μg/mL, in which the concentration of epidermal growth factor, basic fibroblast growth factor and vascular endothelial growth factor were respectively (470.625±2.546), (4.121±0.026) and (0.172±0.002) ng/L. At 1-7 days, the cellproliferation rate of the 10%and 15%concentration groups was greater than that in 20%and 25%concentration groups (P<0.05);10%and 15%concentrations promoted cellproliferation, while 20%and 25%concentrations inhibited cellproliferation. There were no significant difference in the viability of Schwann cell96 between the control group and the experimental group (P>0.05). Low concentrations (10%, 15%) of human amniotic homogenate supernatant promote the proliferation of Schwann cell96, while high concentrations (20%, 25%) of human amniotic homogenate supernatant inhibit cellproliferation.