CAJ | 학술논문

背景：神经生长因子在维持中枢神经系统神经细胞的存活、分化，促进其生长、发育，维持其功能方面具有不可替代的作用。 　　目的：观察灯盏花素对SD大鼠大脑皮质体外培养神经元生长的影响，并初步探讨其机制。 　　方法：取新生SD大鼠胚胎，取其大脑皮质后，对神经元进行原代培养，随机分为3组，其中灯盏花素组加入10 g/L灯盏花素，对照组加入等量生理盐水，正常组不作任何处理，各组又分为24 h、48 h亚组。对24孔板中各组细胞各时间点采集图像，之后采用RT-PCR技术检测神经生长因子、酪氨酸激酶A mRNA的表达变化。对96孔板中各组细胞不同时间点采用MTT检测神经元生长活力。 　　结果与结论：正常组和对照组在各时间点细胞数、胞体面积、突起长度及细胞活力无明显差异(P>0.05)，但正常组及对照组均有随时间点延长，3个指标均上调(P<0.05)，灯盏花素组细胞数、胞体面积、突起长度及细胞活力均较正常组及对照组升高(P<0.05)。神经生长因子及TrkA mRNA的表达在正常组和对照组在各时间点无明显差异(P>0.05)，灯盏花素组各时间点较正常组和对照组上调(P<0.05)。提示灯盏花素促进SD大鼠脑源性神经元的存活、生长，其机制可能是通过上调神经生长因子及其受体TrkA的表达发挥作用。
배경：신경생장인자재유지중추신경계통신경세포적존활、분화，촉진기생장、발육，유지기공능방면구유불가체대적작용。 　　목적：관찰등잔화소대SD대서대뇌피질체외배양신경원생장적영향，병초보탐토기궤제。 　　방법：취신생SD대서배태，취기대뇌피질후，대신경원진행원대배양，수궤분위3조，기중등잔화소조가입10 g/L등잔화소，대조조가입등량생리염수，정상조불작임하처리，각조우분위24 h、48 h아조。대24공판중각조세포각시간점채집도상，지후채용RT-PCR기술검측신경생장인자、락안산격매A mRNA적표체변화。대96공판중각조세포불동시간점채용MTT검측신경원생장활력。 　　결과여결론：정상조화대조조재각시간점세포수、포체면적、돌기장도급세포활력무명현차이(P>0.05)，단정상조급대조조균유수시간점연장，3개지표균상조(P<0.05)，등잔화소조세포수、포체면적、돌기장도급세포활력균교정상조급대조조승고(P<0.05)。신경생장인자급TrkA mRNA적표체재정상조화대조조재각시간점무명현차이(P>0.05)，등잔화소조각시간점교정상조화대조조상조(P<0.05)。제시등잔화소촉진SD대서뇌원성신경원적존활、생장，기궤제가능시통과상조신경생장인자급기수체TrkA적표체발휘작용。
BACKGROUND:Nerve growth factor plays an irreplaceable role on the survival, differentiation and maintenance of the central nervous system cells, also promote the growth and development of these cells. OBJECTIVE:To explore effect of Breviscapine on the growth of in vitro cultured neurons in cerebral cortex of Sprague-Dawley rats, and preliminarily investigate the action mechanism. METHODS:The cerebral cortex of newborn Sprague-Dawley rat embryos was col ected, the neurons were primary cultured and randomly divided into three groups:normal control group (no treatment), control group (neurons were cultured with normal saline), Breviscapine group (neurons were cultured with 10 g/L Breviscapine). Furthermore each group was assigned into 24-hour and 48-hour subgroups. Images were captured from the 24-wel plates at each time points. RT-PCR was applied to examine the expression of nerve growth factor and TrkA mRNA. MTT was used to detect the neuronal growth at each time point. RESULTS AND CONCLUSION:There was no difference between normal control group and control group in the cellcounts, cellbody area, neurite length and viability (P>0.05), as the time prolonged, al data were raised in the two groups (P<0.05). Breviscapine group showed a higher cellcount, cellbody area, neurite length and viability than normal control group and control group (P<0.05). No significant difference was found in the expression of nerve growth factor and TrkA mRNA between normal control group and control group (P>0.05). At each time point, these data in Breviscapine group were increased compared with normal control group and control group (P<0.05). Breviscapine can promote the survival and growth of brain-derived neurons in Sprague-Dawley rats, and the mechanism might depend on the up-regulating expression of nerve growth factor and its receptor TrkA.