中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
20期
3184-3189
,共6页
组织构建%组织工程%口腔组织构建%体外培养%人牙周膜细胞%碱性成纤维细胞生长因子
組織構建%組織工程%口腔組織構建%體外培養%人牙週膜細胞%堿性成纖維細胞生長因子
조직구건%조직공정%구강조직구건%체외배양%인아주막세포%감성성섬유세포생장인자
periodontal ligament%cells%fibroblast growth factor 2%cellproliferation
背景：碱性成纤维细胞生长因子是具有多功能的细胞生长因子，对来源于中胚层及神经外胚层的细胞有明显的促进增殖作用。<br> 目的：观察碱性成纤维细胞生长因子对体外培养人牙周膜细胞的作用。<br> 方法：将第5代人牙周膜细胞，以1×108 L-1的浓度分别接种到96孔板，随机分成4组，分别加入含0，1，10，100μg/L的碱性成纤维细胞生长因子、体积分数为15%胎牛血清的α-MEM培养基进行培养。在第1，3，5，7天测定细胞的增殖情况，在第1，7天检测碱性磷酸酶活性。<br> 结果与结论：4组之间人牙周膜细胞增殖情况的差异有显著性意义(F=6.586，P=0.024)，随着碱性成纤维细胞生长因子质量浓度的增大，吸光度值均增大，其中100μg/L碱性成纤维细胞生长因子组的吸光度值均大于其他组(P <0.05)；碱性成纤维细胞生长因子各组的碱性磷酸酶活性均低于对照组(P=0.000)，浓度越大，活性越低(P <0.05)。结果显示碱性成纤维细胞生长因子在1-100μg/L范围内质量浓度越高，促进人牙周膜细胞增殖和抑制碱性磷酸酶活性的作用越强。
揹景：堿性成纖維細胞生長因子是具有多功能的細胞生長因子，對來源于中胚層及神經外胚層的細胞有明顯的促進增殖作用。<br> 目的：觀察堿性成纖維細胞生長因子對體外培養人牙週膜細胞的作用。<br> 方法：將第5代人牙週膜細胞，以1×108 L-1的濃度分彆接種到96孔闆，隨機分成4組，分彆加入含0，1，10，100μg/L的堿性成纖維細胞生長因子、體積分數為15%胎牛血清的α-MEM培養基進行培養。在第1，3，5，7天測定細胞的增殖情況，在第1，7天檢測堿性燐痠酶活性。<br> 結果與結論：4組之間人牙週膜細胞增殖情況的差異有顯著性意義(F=6.586，P=0.024)，隨著堿性成纖維細胞生長因子質量濃度的增大，吸光度值均增大，其中100μg/L堿性成纖維細胞生長因子組的吸光度值均大于其他組(P <0.05)；堿性成纖維細胞生長因子各組的堿性燐痠酶活性均低于對照組(P=0.000)，濃度越大，活性越低(P <0.05)。結果顯示堿性成纖維細胞生長因子在1-100μg/L範圍內質量濃度越高，促進人牙週膜細胞增殖和抑製堿性燐痠酶活性的作用越彊。
배경：감성성섬유세포생장인자시구유다공능적세포생장인자，대래원우중배층급신경외배층적세포유명현적촉진증식작용。<br> 목적：관찰감성성섬유세포생장인자대체외배양인아주막세포적작용。<br> 방법：장제5대인아주막세포，이1×108 L-1적농도분별접충도96공판，수궤분성4조，분별가입함0，1，10，100μg/L적감성성섬유세포생장인자、체적분수위15%태우혈청적α-MEM배양기진행배양。재제1，3，5，7천측정세포적증식정황，재제1，7천검측감성린산매활성。<br> 결과여결론：4조지간인아주막세포증식정황적차이유현저성의의(F=6.586，P=0.024)，수착감성성섬유세포생장인자질량농도적증대，흡광도치균증대，기중100μg/L감성성섬유세포생장인자조적흡광도치균대우기타조(P <0.05)；감성성섬유세포생장인자각조적감성린산매활성균저우대조조(P=0.000)，농도월대，활성월저(P <0.05)。결과현시감성성섬유세포생장인자재1-100μg/L범위내질량농도월고，촉진인아주막세포증식화억제감성린산매활성적작용월강。
BACKGROUND:Basic fibroblast growth factor is a pluripotent cytokine that can promote the proliferation of mesodermal and neuroectodermal cells. <br> OBJECTIVE:To observe the effect of basic fibroblast growth factor in human periodontal ligament cells cultured in vitro. <br> METHODS:Human periodontal ligament cells at passage 5 were inoculated into the 96-wel plates at the density of 1×108/L, and were randomly divided into four groups. The cells were cultured inα-MEM containing 15%fetal bovine serum and 0, 1, 10, 100μg/L basic fibroblast growth factor, respectively. At 1, 3, 5, 7 days of the culture, the cellproliferation was determined, and the activity of alkaline phosphates was detected at 1 and 7 days. <br> RESULTS AND CONCLUSION:There were significant differences in the proliferation of human periodontal ligament cells among the four groups (F=6.586, P=0.024). As the increase of the basic fibroblast growth factor concentrations, the absorbance value was gradual y increased and reached the peak in 100μg/L basic fibroblast growth factor group (P<0.05). The alkaline phosphatase activity in basic fibroblast growth factor groups was lower than that of the control group (P=0.000), the higher the concentration was, the lower activity was (P<0.05). Results show that basic fibroblast growth factor can promote the proliferation of human periodontal ligament cells and inhibit the activity of alkaline phosphatase, and the effect is concentration-dependent.
