CAJ | 학술논문

背景：近几年来研究发现内质网应激导致细胞凋亡在细胞缺血性损害中起重要作用，成为缺血心肌的研究热点。下肢缺血预处理对未成熟心肌具有明显保护作用，但下肢缺血预处理对未成熟心肌内质网应激细胞凋亡是否存在影响至今未知。 　　目的：探讨下肢缺血预处理对未成熟心肌内质网应激和细胞凋亡的影响。 　　方法：采用兔离体心脏Langendorff灌注模型，24只幼兔随机分为3组：对照组仅灌注KH液180 min；缺血再灌注组心脏灌注20 min后，停灌60 min，复灌100 min；下肢缺血预处理组，反复3次阻断双下肢血流5 min/放5 min，建立Langendorff模型，然后重复缺血再灌注组方法。TUNEL法检测心肌细胞凋亡率、Western blot检测GRP78、Bcl-2、Bax和Fas蛋白表达。 　　结果与结论：下肢缺血预处理组与缺血再灌注组比较，心肌细胞凋亡率明显减少；Bcl-2表达明显增多，GRP78、Bax、Fas表达明显减少。结果表明下肢缺血预处理可能通过调控内质网过度应激GRP78表达以及Bcl-2、Bax、Fas蛋白的表达调控心肌细胞凋亡。
배경：근궤년래연구발현내질망응격도치세포조망재세포결혈성손해중기중요작용，성위결혈심기적연구열점。하지결혈예처리대미성숙심기구유명현보호작용，단하지결혈예처리대미성숙심기내질망응격세포조망시부존재영향지금미지。 　　목적：탐토하지결혈예처리대미성숙심기내질망응격화세포조망적영향。 　　방법：채용토리체심장Langendorff관주모형，24지유토수궤분위3조：대조조부관주KH액180 min；결혈재관주조심장관주20 min후，정관60 min，복관100 min；하지결혈예처리조，반복3차조단쌍하지혈류5 min/방5 min，건립Langendorff모형，연후중복결혈재관주조방법。TUNEL법검측심기세포조망솔、Western blot검측GRP78、Bcl-2、Bax화Fas단백표체。 　　결과여결론：하지결혈예처리조여결혈재관주조비교，심기세포조망솔명현감소；Bcl-2표체명현증다，GRP78、Bax、Fas표체명현감소。결과표명하지결혈예처리가능통과조공내질망과도응격GRP78표체이급Bcl-2、Bax、Fas단백적표체조공심기세포조망。
BACKGROUND:In recent years, endoplasmic reticulum stress-caused apoptosis plays a crucial role in ischemia impairment and has become the hotspot of studies addressing myocardial ischemia/reperfusion (I/R) injury. The lower limb ischemic preconditioning (LIP) has the obvious protective effect on the immature myocardium, but until now, no study reports whether LIP effects on endoplasmic reticulum stress apoptosis in immature myocardial cells. OBJECTIVE:To investigate the effect of LIP on endoplasmic reticulum stress and apoptosis. METHODS:Langendorff-perfused isolated rabbit hearts were used in this study. Twenty-four immature rabbits were randomized into three groups. Control group:Isolated rabbit heart was only perfused with Krebs-Henseleit for 180 minutes. I/R group:Isolated rabbit heart was perfused 20 minutes, and then ischemia for 60 minutes fol owed by reperfusion 100 minutes. LIP group:Limbs were repeatedly obstructed 5 minutes and relaxed 5 minutes for three times, to establish Langendorff models, and then repeated the method of ischemia/reperfusion in I/R group. The myocardial apoptosis was assayed with TUNEL method. The expression of glucose-regulated protein 78, Bcl-2, Bax and Fas was detected with western blot analysis. RESULTS AND CONCLUSION:Compared with I/R group, apoptosis rate was significantly lower, the expression of Bcl-2 was significantly higher, and the expression of glucose-regulated protein 78, Bax and Fas was significantly lower in LIP group. This study demonstrated that LIP regulates myocardial cellapoptosis through reducing the expression of endopasmic reticulum stress GRP78, Bax and Fas and increasing the expression of Bcl-2.