CAJ | 학술논문

背景：健康新生儿脐带胶是一种无血管的黏液结缔组织，有一定的弹性和韧性，含有溶菌酶、胎盘球蛋白、透明质酸酶、AMP抗体和补体系统，不容易发生感染和排斥反应，还含有较多的间充质干细胞，临床已被用于眼部整形手术。 　　目的：观察脐带胶作为睑板替代物在豚鼠上眼睑重建中的组织相容性和组织病理变化。 　　方法：取健康新生儿脐带中的黏液结缔组织-脐带胶，制备成厚薄相近的组织块；同时制作豚鼠睑板全层缺损模型并植入脐带胶组织块，观察动物大体情况，分别于脐带胶植入后1，2，3周取其交界处眼睑组织，光镜下观察组织学改变。 　　结果与结论：所有动物在植入脐带胶材料后均未发生感染、脱位等情况，所有动物角膜上皮完整，无角膜上皮脱落。脐带胶代替睑板植入后1周时，结膜红润，切口无红肿等排斥反应发生，角膜上皮完整，眼睑运动可；脐带胶与肌肉间的炎性细胞浸润。植入后2周时结膜面红润，切口无红肿，无分泌物增多等排斥反应发生，角膜上皮完整，眼睑及眼球运动良好，无睑球粘连发生；脐带胶有溶解吸收倾向，炎性细胞不明显。植入3周时结膜切口愈合良好，角膜上皮完整，眼睑柔软，形态与对侧眼无明显区别；脐带胶部分吸收，其周边有新的纤维生成，无炎性细胞。说明脐带胶的免疫原性低，可引导新生胶原的生长，是一种良好的睑板替代物。
배경：건강신생인제대효시일충무혈관적점액결체조직，유일정적탄성화인성，함유용균매、태반구단백、투명질산매、AMP항체화보체계통，불용역발생감염화배척반응，환함유교다적간충질간세포，림상이피용우안부정형수술。 　　목적：관찰제대효작위검판체대물재돈서상안검중건중적조직상용성화조직병리변화。 　　방법：취건강신생인제대중적점액결체조직-제대효，제비성후박상근적조직괴；동시제작돈서검판전층결손모형병식입제대효조직괴，관찰동물대체정황，분별우제대효식입후1，2，3주취기교계처안검조직，광경하관찰조직학개변。 　　결과여결론：소유동물재식입제대효재료후균미발생감염、탈위등정황，소유동물각막상피완정，무각막상피탈락。제대효대체검판식입후1주시，결막홍윤，절구무홍종등배척반응발생，각막상피완정，안검운동가；제대효여기육간적염성세포침윤。식입후2주시결막면홍윤，절구무홍종，무분비물증다등배척반응발생，각막상피완정，안검급안구운동량호，무검구점련발생；제대효유용해흡수경향，염성세포불명현。식입3주시결막절구유합량호，각막상피완정，안검유연，형태여대측안무명현구별；제대효부분흡수，기주변유신적섬유생성，무염성세포。설명제대효적면역원성저，가인도신생효원적생장，시일충량호적검판체대물。
BACKGROUND:Healthy human umbilical cord jel y is a mucous connective tissue without vessels and has been used in eye plastic operation because of its elasticity and toughness. It contains lysozyme, placental globulin, hyaluronic acid enzyme, AMP antibody and complement, and also contains a lot of mesenchymal stem cells, so it is not prone to infection and rejection. OBJECTIVE:To observe the histocompatibility and histopathological changes of human umbilical cord jel y as a tarsal substitute transplanted for eyelid reconstruction in guinea pigs. METHODS:The mucous connective tissue of healthy neonate umbilical cord jel y was made into tissue blocks at even thickness. Models of tarsal defects were established in guinea pigs, and then the mucous connective tissue of healthy neonate umbilical cord jel y was implanted. Samples of implanted materials were col ected for histological examination at 1, 2, 3 weeks postoperation under light microscope. RESULTS AND CONCLUSION:There were no obvious rejection, infection and eyelid deformation observed, the corneas of al the animals were clear, corneal epithelial shedding did not occur, and the eyelid could move normal y. One week after implantation, there was no infection and rejection on the conjunctiva and the incision of the eyelid, the eyelid could move normal y, and partial inflammatory cells were observed between the human umbilical cord jel y and the muscle of the eyelid with microscopy. Two weeks after implantation, there was no infection and rejection on the conjunctiva and the incision of the eyelid, the cornea was clear, the eyelid and eye could move normal y, and no symblepharon occurred;umbilical cord jel y showed the tendency of absorption, and the inflammatory cells were reduced at 2 weeks after implantation. Three weeks after implantation, the incision of the conjunctiva healed wel , the cornea was clear;there was no difference in the eyelid form and movement between the two eyes;the umbilical cord jel y was absorbed partial y, normal fibrous tissues formed and there were no inflammatory cells. With low immunogenicity, human umbilical cord jel y can guide the growth of new col agen and serve as an ideal tarsal substitute.