中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
21期
3355-3360
,共6页
鲁锋%严雪征%马术友%张鸿图
魯鋒%嚴雪徵%馬術友%張鴻圖
로봉%엄설정%마술우%장홍도
生物材料%缓释材料%骨生物材料%局部缓释系统%骨髓炎%加替沙星%聚癸二酸酐%缓释%生物相容性%骨膜反应%骨破坏%脓肿%骨感染
生物材料%緩釋材料%骨生物材料%跼部緩釋繫統%骨髓炎%加替沙星%聚癸二痠酐%緩釋%生物相容性%骨膜反應%骨破壞%膿腫%骨感染
생물재료%완석재료%골생물재료%국부완석계통%골수염%가체사성%취계이산항%완석%생물상용성%골막반응%골파배%농종%골감염
osteomyelitis%biocompatible materials%infection%femur
背景：聚酸酐材料具有表面溶蚀性、生物可降解性和释药速率可调性，为人体药物缓释材料，但关于聚癸二酸酐缓释系统的应用至今少有报道。
<br> 目的：观察加替沙星-聚癸二酸酐局部缓释系统对兔股骨骨髓炎感染模型的预防作用。
<br> 方法：按 Norden法将30只兔制作股骨金黄色葡萄球菌骨髓炎兔模型，分别用加替沙星-聚癸二酸酐缓释制剂和加替沙星进行干预，并与模型组作对照，共分3组，每组10只，观察建模后兔一般情况、解剖学观察、X射线片表现、骨培养及细菌计数、组织学检查。
<br> 结果与结论：建模后8周，模型组兔建模后，食欲差，皮毛不光滑，体质量减轻，3只兔于3周内死亡，其他7只X射线片上骨膜反应较重，可见小块死骨形成，解剖学检查均可见脓肿形成，骨培养及组织学检查均可见骨髓炎表现；加替沙星组建模后体质量减轻，2只兔于建模后3周内死亡，解剖学检查可见有4只动物有脓肿形成，X射线片上可见8只动物骨膜反应较轻，未见死骨形成，骨培养及组织学检查6只兔有骨髓炎表现；加替沙星-聚癸二酸酐缓释制剂治疗组兔一般情况好，解剖学检查未见异常，X射线片检查、骨培养和细菌计数、组织学检查均未见骨髓炎表现。结果证实，加替沙星-聚癸二酸酐局部缓释制剂预防股骨骨髓炎感染效果较好。
揹景：聚痠酐材料具有錶麵溶蝕性、生物可降解性和釋藥速率可調性，為人體藥物緩釋材料，但關于聚癸二痠酐緩釋繫統的應用至今少有報道。
<br> 目的：觀察加替沙星-聚癸二痠酐跼部緩釋繫統對兔股骨骨髓炎感染模型的預防作用。
<br> 方法：按 Norden法將30隻兔製作股骨金黃色葡萄毬菌骨髓炎兔模型，分彆用加替沙星-聚癸二痠酐緩釋製劑和加替沙星進行榦預，併與模型組作對照，共分3組，每組10隻，觀察建模後兔一般情況、解剖學觀察、X射線片錶現、骨培養及細菌計數、組織學檢查。
<br> 結果與結論：建模後8週，模型組兔建模後，食欲差，皮毛不光滑，體質量減輕，3隻兔于3週內死亡，其他7隻X射線片上骨膜反應較重，可見小塊死骨形成，解剖學檢查均可見膿腫形成，骨培養及組織學檢查均可見骨髓炎錶現；加替沙星組建模後體質量減輕，2隻兔于建模後3週內死亡，解剖學檢查可見有4隻動物有膿腫形成，X射線片上可見8隻動物骨膜反應較輕，未見死骨形成，骨培養及組織學檢查6隻兔有骨髓炎錶現；加替沙星-聚癸二痠酐緩釋製劑治療組兔一般情況好，解剖學檢查未見異常，X射線片檢查、骨培養和細菌計數、組織學檢查均未見骨髓炎錶現。結果證實，加替沙星-聚癸二痠酐跼部緩釋製劑預防股骨骨髓炎感染效果較好。
배경：취산항재료구유표면용식성、생물가강해성화석약속솔가조성，위인체약물완석재료，단관우취계이산항완석계통적응용지금소유보도。
<br> 목적：관찰가체사성-취계이산항국부완석계통대토고골골수염감염모형적예방작용。
<br> 방법：안 Norden법장30지토제작고골금황색포도구균골수염토모형，분별용가체사성-취계이산항완석제제화가체사성진행간예，병여모형조작대조，공분3조，매조10지，관찰건모후토일반정황、해부학관찰、X사선편표현、골배양급세균계수、조직학검사。
<br> 결과여결론：건모후8주，모형조토건모후，식욕차，피모불광활，체질량감경，3지토우3주내사망，기타7지X사선편상골막반응교중，가견소괴사골형성，해부학검사균가견농종형성，골배양급조직학검사균가견골수염표현；가체사성조건모후체질량감경，2지토우건모후3주내사망，해부학검사가견유4지동물유농종형성，X사선편상가견8지동물골막반응교경，미견사골형성，골배양급조직학검사6지토유골수염표현；가체사성-취계이산항완석제제치료조토일반정황호，해부학검사미견이상，X사선편검사、골배양화세균계수、조직학검사균미견골수염표현。결과증실，가체사성-취계이산항국부완석제제예방고골골수염감염효과교호。
BACKGROUND:Polyanhydride materials characterized as surface corrosion resistance, biodegradability and adjustable release rate have been used as drug delivery materials for the body, but the application of polysebacic anhydride delivery system has been rarely reported.
<br> OBJECTIVE:To study the role of gatifloxa-polysebacic anhydride local delivery system in the prevention of osteomyelitis in rabbits.
<br> METHODS:According to Norden method, we made Staphylococcus aureus-induced osteomyelitis models in the femur of 30 rabbits. There were three groups in the study, 10 rats in each group. Gatifloxa-polysebacic anhydride local delivery system and gatifloxa were respectively used in comparison with model group. After modeling, general observation, anatomical observation, X-ray examination, bone culture and bacteria counting, and histological examination were performed.
<br> RESULTS AND CONCLUSION:After 8 weeks, animals in the model group presented with poor appetite, rough fur, and weight loss. Among these 10 rabbits, 3 rabbits died within 3 weeks, and another 7 rabbits appeared to have severe periosteal reactions on the X-ray films, that is, abscess formation shown in the anatomical examination and visible manifestation of osteomyelitis through bone culture and histological examination. In the gatifloxa group, two rabbits died within 3 weeks, abscess formation was found in four rabbits, and periosteal reactions were mild without bone sequestration. Six of 10 rabbits in the gatifloxa appeared to have manifestation of osteomyelitis. In the delivery system group, the animals had a good general state of health and no abnormality was found in the anatomical examination, X-ray examination, bone culture and bacteria count, and histological examination. These findings indicate that the gatifloxa-polysebacic anhydride local sustained release preparation could effectively prevent osteomyelitis.
