CAJ | 학술논문

背景：RADA16是较成熟的自组装纳米短肽材料，在亲水面往复形成互补离子键，可组装为纳米纤维，并且能够促MC3T3 E1细胞的黏附、伸展和增殖。 　　目的：观察新型自组装多肽水凝胶NBD/RADA16对小鼠前成骨细胞MC3T3 E1成骨分化能力的影响。 　　方法：将MC3T3 E1细胞分别接种于自组装多肽水凝胶NBD/RADA16与RADA16水凝胶中，进行成骨诱导培养，以单纯成骨诱导培养的细胞为对照。诱导培养1，3，6 d检测细胞碱性磷酸酶活性；诱导培养7 d后， Western Blot检测细胞骨形态发生蛋白2的表达；诱导培养21 d后，茜素红染色观察细胞钙化结节。 　　结果与结论：MC3T3 E1细胞在NBD/RADA16多肽水凝胶上生长状态良好，优于在RADA16上生长的细胞。自组装多肽水凝胶NBD/RADA16上MC3T3 E1细胞的碱性磷酸酶活性高于RADA16水凝胶上及单纯成骨诱导培养的细胞(P<0.01)。自组装多肽水凝胶NBD/RADA16上MC3T3 E1细胞的矿化基质沉积、骨形态发生蛋白2表达高于RADA16水凝胶上的细胞(P<0.01)。结果提示NBD/RADA16自组装多肽水凝胶较RADA16水凝胶更能促进MC3T3 E1细胞的成骨分化。
배경：RADA16시교성숙적자조장납미단태재료，재친수면왕복형성호보리자건，가조장위납미섬유，병차능구촉MC3T3 E1세포적점부、신전화증식。 　　목적：관찰신형자조장다태수응효NBD/RADA16대소서전성골세포MC3T3 E1성골분화능력적영향。 　　방법：장MC3T3 E1세포분별접충우자조장다태수응효NBD/RADA16여RADA16수응효중，진행성골유도배양，이단순성골유도배양적세포위대조。유도배양1，3，6 d검측세포감성린산매활성；유도배양7 d후， Western Blot검측세포골형태발생단백2적표체；유도배양21 d후，천소홍염색관찰세포개화결절。 　　결과여결론：MC3T3 E1세포재NBD/RADA16다태수응효상생장상태량호，우우재RADA16상생장적세포。자조장다태수응효NBD/RADA16상MC3T3 E1세포적감성린산매활성고우RADA16수응효상급단순성골유도배양적세포(P<0.01)。자조장다태수응효NBD/RADA16상MC3T3 E1세포적광화기질침적、골형태발생단백2표체고우RADA16수응효상적세포(P<0.01)。결과제시NBD/RADA16자조장다태수응효교RADA16수응효경능촉진MC3T3 E1세포적성골분화。
BACKGROUND:RADA16 is a mature amphiphilic self-assembling peptide, which can be assembled into nanofibers, and promote MC3T3 E1 cellattachment, spreading and proliferation. OBJECTIVE:To observe the effects of the new-type self-assembling peptide hydrogel NBD/RADA16 on osteogenic differentiation of mouse preosteoblasts MC3T3 E1. METHODS:The MC3T3 E1 cells were inoculated in NBD/RADA16 self-assembling peptide hydrogel and RADA16 hydrogel for osteogenic induction. cells undergoing simple osteogenic induction served as controls. After culture for 1, 3, 6 days, the activity of alkaline phosphatase was detected. After 7 days, western blot assay was used to determine the expression of bone morphogenetic protein-2. After 21 days, alizarin red staining was used to observe calcified nodules. RESULTS AND CONCLUSION:MC3T3 E1 cells grew wel on the NBD/RADA16 peptide hydrogel, which were superior to those on the RADA16 hydrogel. The activity of alkaline phoshpatase was higher in the NBD/RADA16 group than the RADA16 and control groups (P<0.01). Compared with the RADA16 hydrogel, mineralized matrix deposition and expression of bone morphogenetic protein-2 were higher on the NBD/RADA16 peptide hydrogel (P<0.01). These findings indicate that the NBD/RADA16 peptide hydrogel is superior to the RADA16 hydrogel for promoting the osteogenic differentiation of MC3T3 E1 cells.