CAJ | 학술논문

背景：海藻酸钠和壳聚糖分别是聚阳离子和聚阴离子的天然高分子材料，二者可以互为交联剂形成复合凝胶，避免使用普通交联剂产生的细胞毒性。 　　目的：制备海藻酸钠壳聚糖复合凝胶并评价其体外细胞毒性。 　　方法：以0.25 mol/L乙酸溶解壳聚糖，制成质量浓度30 g/L溶液，再以0.1 mol/L的NaOH中和酸性得到壳聚糖絮状沉淀，将壳聚糖絮状沉淀与质量浓度3%海藻酸钠等比例混合，高频振动混匀，使二者形成复合凝胶。使用傅里叶变换红外光谱、扫描电镜分析观察凝胶成分和交联纤维网络结构。分别以海藻酸钠壳聚糖复合凝胶24，72 h浸提液、聚乙烯24，72 h浸提液及苯酚溶液培养L-929细胞，体外检测其细胞毒性。 　　结果与结论：傅里叶变换红外光谱检测到海藻酸钠壳聚糖复合凝胶特征性峰值改变，扫描电镜显示其内部形成丰富间隙的空间网络结构。浸提液法检测海藻酸钠壳聚糖复合凝胶的细胞毒性为合格，表明海藻酸钠/壳聚糖复合凝胶具有成为组织工程支架材料的良好条件。
배경：해조산납화각취당분별시취양리자화취음리자적천연고분자재료，이자가이호위교련제형성복합응효，피면사용보통교련제산생적세포독성。 　　목적：제비해조산납각취당복합응효병평개기체외세포독성。 　　방법：이0.25 mol/L을산용해각취당，제성질량농도30 g/L용액，재이0.1 mol/L적NaOH중화산성득도각취당서상침정，장각취당서상침정여질량농도3%해조산납등비례혼합，고빈진동혼균，사이자형성복합응효。사용부리협변환홍외광보、소묘전경분석관찰응효성분화교련섬유망락결구。분별이해조산납각취당복합응효24，72 h침제액、취을희24，72 h침제액급분분용액배양L-929세포，체외검측기세포독성。 　　결과여결론：부리협변환홍외광보검측도해조산납각취당복합응효특정성봉치개변，소묘전경현시기내부형성봉부간극적공간망락결구。침제액법검측해조산납각취당복합응효적세포독성위합격，표명해조산납/각취당복합응효구유성위조직공정지가재료적량호조건。
BACKGROUND:Sodium alginate and chitosan are the polycation and polyanion natural polymer materials respectively, and they can be crosslinking agents complementing each other to form composite gel and avoid the cytotoxicity resulting from some common crosslinking agents . OBJECTIVE:To prepare the chitosan-sodium alginate composite gel and evaluate its cytotoxicity in vitro. METHODS:Chitosan was dissolved in 0.25 mol/L acetic acid to make a 30 g/L mass concentration solution, and 0.1 mol/L NaOH solution was added to neutralize its acidity. Neutralization of the chitosan solutions leads to the formation of a precipitate in ultrasmal particles. Then the chitosan and 3%sodium alginate solution in deionized water were mixed in 1:1 volume ratio by high frequency oscil ating to produce composite gel. The composite gel were detected by scanning electron microscopy and Fourier transform infrared spectrometry after freeze-drying. The 24-hour and 72-hour leaching solutions of composite gel, 24-hour and 72-hour leaching solutions of polyethylene and phenol solution were added to the L-929 cells’ culture medium respectively in order to evaluate the cytotoxicity of composite gel in vitro. RESULTS AND CONCLUSION:The results of Fourier transform infrared spectrometry showed the variation of characteristic peak values of composite gel which were different from sodium alginate and chitosan;and under scanning electron microscope, a spatial network structure formed with abundant intervals. Result of the cytotoxicity valuation was qualified for the chitosan-sodium alginate composite gel. These findings indicate that the chitosan-sodium alginate composite gel can be used as tissue engineering scaffold materials.