CAJ | 학술논문

建立了高效液相色谱-四极杆飞行时间串联质谱快速检测饮料中糖精钠、甜蜜素、安赛蜜、阿斯巴甜、纽甜、三氯蔗糖6种人工合成甜味剂的方法。样品经水提取，采用C18色谱柱，以甲醇和0.1%甲酸-10 mmol/L甲酸铵溶液为流动相，梯度洗脱，四极杆飞行时间串联质谱电喷雾负离子模式检测。各化合物在0.02～2.0 mg/L范围内均呈现良好的线性关系，相关系数均大于0.998。样品平均添加回收率为63.0%～113.2%，测定结果的相对标准偏差均小于9.6%(n=5)。该方法简便快捷，选择性好，灵敏度高，可满足国内外现行法规的限量要求。
건립료고효액상색보-사겁간비행시간천련질보쾌속검측음료중당정납、첨밀소、안새밀、아사파첨、뉴첨、삼록자당6충인공합성첨미제적방법。양품경수제취，채용C18색보주，이갑순화0.1%갑산-10 mmol/L갑산안용액위류동상，제도세탈，사겁간비행시간천련질보전분무부리자모식검측。각화합물재0.02～2.0 mg/L범위내균정현량호적선성관계，상관계수균대우0.998。양품평균첨가회수솔위63.0%～113.2%，측정결과적상대표준편차균소우9.6%(n=5)。해방법간편쾌첩，선택성호，령민도고，가만족국내외현행법규적한량요구。
A new method was established for the determination of 6 synthetic sweeteners (sodium saccharin, cyclamate, acesulfame-K, aspartame, neotame, sucralose) in beverage, by using high performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-Q TOF MS). The samples were extracted by water and measured directly by HPLC-Q TOF MS with electrospray ionization in negative mode. The compounds were separated by a C18 column with methyl alcohol and 0.1% formic acid-10 mmol/L ammonium formate solution as the mobile phase. A detailed fragmentation study for 6 synthentic sweeteners was carried out by time of flight. The 6 compounds behave linearly in the range of 0.02-2.0 mg/L with the correlation coefficient of more than 0.998. The recoveries were 63.0%-113.2%, with coefficients of variation below 9.6%(n=5). The method is suitable for routine qualitative and quantitative analyses of synthetic sweeteners in beverage.